Feasibility Study Of Microenvironment Remodeling In Small-diameter Artificial Blood Vessels

Cardiovascular disease seriously endangers human health,and its common manifestations are coronary heart disease,deep vein thrombosis,and peripheral arterial disease etc.Vascular procedures,such as coronary artery bypass grafting,arteriovenous shunting,and treatment of congenital coronary arteries,require bio responsive vascular substitutes.Limited sources of autografts,artificial vessels are regarded as the best alternative to autologous vessels,but low patency due to shortand medium-term thrombosis limits their clinical use.Artificial polymer materials are prepared from tissue-engineered blood vessels,usually with excellent mechanical properties.However,its histocompatibility needs to be improved,and it is not suitable for developing materials as small-diameter artificial blood vessels alone.The utilization of extracellular matrix modification is expected to create artificial blood vessels with properties similar to natural tissues,capable of providing cells with a growth microenvironment,but it still lacks systematic and in-depth studies.In this study,based on previous work,the method of obtaining extracellular matrix was optimized,and a composite small-diameter artificial blood vessel was fabricated by combining the advantages of the natural characteristics of extracellular matrix and the excellent mechanical properties of artificial tissue engineering scaffolds,which were able to induce the adhesion and proliferation of vascular cells.The artificial blood vessel was subjected to rat abdominal aortic replacement,and the results showed that it could accelerate tissue regeneration and microenvironment remodeling at the transplantation site.This study provides a new method for in vitro reconstruction of the extracellular microenvironment and sheds new light on the development of small diameter tissue-engineered vascular grafts.