Expression And Clinical Analysis Of STK32C Protein In Gallbladder Carcinoma

Objective: Gallbladder carcinoma is extremely malignant,has poor prognosis and low overall survival rate.It is urgent to explore and develop more accurate and effective markers for disease diagnosis.Studies have shown that STK32 C gene expression is abnormal in many tumor tissues and is associated with tumor metastasis and prognosis of patients.However,the role and mechanism of this gene in the occurrence and progression of gallbladder carcinoma remain unclear.This study was the first to investigate the expression trend and prognostic value of STK32 C in gallbladder carcinoma.Method: The abnormal expression of STK32 C in biliary carcinoma was analyzed by the gene expression data of TCGA.The study included 84 patients with gallbladder cancer who underwent surgical resection in the First Affiliated Hospital of China Medical University.STK32 C protein expression was detected in patients with gallbladder cancer using immunohistochemistry.The Chi-square test was performed to examine the relationship between STK32 C expression and clinicopathological characteristics.Kaplan-Meier survival curve was utilized for survival analysis.Multi-factor Cox proportional risk model was constructed for analysis to investigate the prognostic significance of STK32 C expression in gallbladder carcinoma.Result: According to the TCGA data,the expression level of the STK32 C gene in biliary carcinoma tissues was considerably greater than in paracancer tissues.After immunohistochemical staining of gallbladder carcinoma sections,different clinicopathological features of gallbladder carcinoma were compared between STK32 C high expression group and low expression group.The rise in STK32 C was linked to the T stage(P < 0.001),N stage(P = 0.027),clinical stage(P < 0.001),recurrence and metastasis(P < 0.001).Kaplan-Meier survival analysis showed that greater STK32 C expression in gallbladder cancer was related with shorter overall survival(OS)and progression-free survival(RFS)(P < 0.01).STK32 C was found to be an independent prognostic factor for OS and PFS in gallbladder cancer patients in both univariate and multivariate models(P = 0.008 and P = 0.034,respectively).Conclusion: In summary,this study suggests that increased STK32 C expression is substantially related with a poor prognosis of gallbladder cancer and may be regarded an independent risk factor for gallbladder cancer.