Effect Of Berberine On Intestinal Tight Junction Protein In Mice With Adenine-induced Renal Failure

Background and purpose: The precaution of chronic kidney disease(CKD)is increasing every year,a health problem for which all people require extra attention.The gut kidney axis,gut flora,and renal system interactions exist.The increase of intestinal permeability will lead to enterogenous endotoxemia and aggravate kidney damage;At the same time,the increase of intestinal permeability will also lead to the aggravation of the body’s oxidative stress state and further destroy the intestinal barrier function.Therefore,it is of great significance to study the drugs that regulate the intestinal barrier for the treatment of CKD.Berberine is an alkaloid extracted from the plant scutellaria baicalensis,which has a variety of pharmacological activities [5].At present,studies have found that berberine has protective effects on glomerular and tubular cell damage.In the model of chronic renal failure,adenine can induce renal tubular injury,renal interstitial fibrosis and other renal damage in mice [6].The intestinal flora stimulates the renal tubular epithelial cells of mice through metabolites,resulting in renal tubular injury [7],and the destruction of intestinal barrier function is closely related to the imbalance of intestinal flora.Previous work shows that berberine can improve renal function by adjusting the flora [7].From the perspective of observing the regulatory effect of berberine on intestinal tight junction protein and the improvement of renal function in mice with renal failure,this paper provides scientific basis for berberine to improve renal function by improving intestinal mucosal barrier.The report is as follows.Research methods:1.Selecting twenty-seven six-week-old C57BL/6 mice,five were selected as the control group,and 22 were selected as the model group.They were fed with 0.2% adenine and normal diet,and the state of mice was closely observed for 8weeks.Two mice were selected by random sampling method,and the changes of mice kidney were observed by HE staining and Masson staining,which confirmed that the model had been successfully established.The remaining 3 mice in the control group were designated as the control group(Group A),and 20 mice with renal failure were divided into 0.1% berberine group(Group B),0.2% berberine group(Group C),0.3% berberine group(Group D),inulin group(Group E),berberine+inulin group(Group F),and renal failure group(Group G)by random number table.2.The mice in group A were given a common diet,group G was given a common diet plus 0.2% adenine,group BCD were given a common diet plus 0.1%,0.2%,and 0.3% berberine,group E was given a common diet plus 5% short-chain inulin,and group F was given 0.2% berberine and 5%short-chain inulin successively for 8 weeks.3.The serum creatinine(Scr)and urea nitrogen(UN)of mice were measured by biochemical instrument;The serum lipopolysaccharide(LPS)of mice was detected by enzyme-linked immunosorbent assay(ELISA);The pathologic changes of mouse kidney were observed by HE staining and Masson staining;Immunohistochemical method was used to find the representation of ZO-1 and Occludin in intestinal tissue.Use Image Pro Plus6.0 software to analyze the photos and measure the average optical density to compare the positive expression of protein.Result: 1.The results of this experiment showed that the protein expression of all three BCF groups was higher than group G(p<0.05),and the expression level of ZO-1 protein in group C was the most significant(p<0.05);There was no significant difference between inulin group and renal failure group;Compared with group G,the expression of Occludin protein in each group was raised(p<0.05).2.The serum creatinine,urea and LPS showed that the levels of serum creatinine,urea and LPS in Group G were significantly higher than those in Group A,while the levels of serum creatinine,urea and LPS in other groups were significantly lower than those in Group G,with significant difference(p<0.05).Unfortunately.The renal HE and Masson staining results showed that the inflammatory cell infiltration and renal fibrosis were more obvious in group G mice than in group A mice,and the renal pathological changes in each drug group were better than those in group G,while the pathological changes in group C were the least.Conclusion: Berberine can significantly reduce the levels of urea nitrogen and serum creatinine in mice with renal failure,and improve renal pathological damage;Berberine can enhance the expression of tight junction proteins,including ocidin and ZO-1,in intestinal tissue of mice.