Study Of Lipid Droplets And Related Proteins In Photodamaged Skin

Objective:High-level ultraviolet radiation is the main reason for environmental stress.Skin overexposure to UV can cause DNA damage and activated oxygen(ROS)accumulation,which is the cause of showing sunburn,aging,skin cancer and other manifestations.Recent studies have shown that fat droplets during stress are critical to protect cell integrity and function:they areolate potential toxic lipids and proteins,maintain energy and oxidation and balance,maintain the balance of membrane and cytoles,regulate self-self Step,provide lipid as a signal medium.Perilipin 2(Perilipin 2,Plin2)is also known as fat differentiation-related proteins,which is not only closely related to oxidation stress,but also plays a crucial part in the balance of lipid droplets and lipid outflow.C/EBPA(CCAAT/Enhancer-Binding Protein-A)is one of the six members of the alkaline brightened zipper transcription factor C/EBP family.Bone marrow cells of alveolar epithelial cells and hematopoietic systems,the differences in expressive spectrum determine their important and unique role in different tissues and organs.Among the oxytic satellite cells treated with oleic acid,CEBPA and Plin2 express their consistency,accompanied by gathered gathered.Jaspar predicts that CEBPA can be a promoter of Plin2,and the above indicates that there are close connections between CEBPA,Plin2,and fat droplets.With the deepening of scientific exploration and the increasing number of research on fat drops,this article uses the scientific measurement system to evaluate the relevant documents of the drip to analyze its hotspots and current conditions.By constructing a photodamage model,lipid droplets and related protein changes were explored.Research methods:In terms of literature:include English literature related to fat drops from January 1st,2013 to December 31,2022 in the core database of the web of science.The literature measurement platform and VosViewer are used for scientific measurement and visual analysis.Experimental aspects:1.This study selected 30 C57BL/6 female mice,randomly divided into 5 groups,different doses of UVA continuously exposed the skin on the back of the mouse for pathological materials.Organizational changes,use Western blot to detect CEBPPA and PLIN2 changes in mice skin tissue,immunohistochemical detection CEBPPA and PLIN2.2.Select the primary fibroblast(HDF)and embryo fibroblasts(3T3)as experimental objects.The MTS method detects different doses of UVA radiation to change the vitality of fibroblasts,and selects appropriate light intensity to build a light damage model.PCR and Western blot were used to determine the changes of CEBPA and PLIN2,and BODIPY493/503 staining was used to detect the expression of lipid droplets in cells.Results:The time is limited from January 1,2013 to December 31,2022,the theme of fat drops,the refinement of 8980 documents,the research on the research on fat drops has continued to increase,and the circulation of the annual articles will not decrease.In global research related to fat drops,China is leading positions,not only the total number of documents is far ahead,but also actively participate in national/regional cooperation.Journal PLOS One,Scientific Reports,International Journal of Molecular Sciences annual publishing publications are relatively high,and McClements DJ has extraordinary achievements in the field of drip research.48 keywords are determined according to the H index,which is mostly connected with metabolic diseases and stress.1.HE results show that different doses of UVA to different degrees of thickening the epidermis caused by different doses of mouse tissue damage.The total dose of 300J is selected for subsequent experiments.2.western blot indicates that UVA can cause an increase in the expression of CEBPPA and PLIN2 in mouse organizations.The dosage is as dosage when the exposure dose is less than 300J,and it is higher than the unscorpoted light group.The result of immunohistochemicals shows that the UVA has increased the expression of the CEBPA and PLIN2 of mice.3.Different doses of UVA different degrees of damage to the primary fibroblasts of human beings.According to MTS results and cell forms,22.5J/CM2 UVA is used for subsequent experiments.4.After UVA irradiation,the level of RNA,CEBPA and PLIN2,the original fibroblasts of human beings,has increased significantly.UVA irradiation can significantly increase the protein level of CEBPPA,and the Bodipy 493/503 visible ultraviolet radiation can increase the aggregation of cell fat droplets.5.After irradiation of different doses of UVA,22.5J/CM2 is selected for subsequent experiments for changes in the vitality of fibroblasts in mice.6.After UVA irradiation,the level of RNA,CEBPA,and PLIN2 of mice into fibroblasts,increased significantly.UVA irradiation can significantly increase the protein level of CEBPPA,which has no effect on the overall expression level of PLIN2.Bodipy 493/503 Dye visible ultraviolet radiation increases the agglomeration of cell fat droplets.Conclusion:1.The global research on lipid droplets is unevenly distributed,and the research enthusiasm has been steadily increasing,especially the research and exploration of the relationship between oxidative stress,autophagy and inflammation outside the function of lipid storage is increasing.2.Increased expression of CEBPA and PLIN2 proteins in UVA-induced mouse skin photoinjury models.3.UVA induces increased expression of CEBPA and PLIN2 in skin fibroblasts,and correspondingly increased the aggregation of lipid droplets in cells.