The Effects Of Sphingosine 1-Phosphate Receptor-2 (S1PR2) On The Biological Behavior Of HepG2 Cells And The Experimental Study On The Effect Of S1PR2 On Mouse Liver Cancer Model

Background: In China,primary liver cancer is a frequent malignant malignancy,ranking fourth among all cancer diseases and second in death rates among cancer patients.The latest global cancer burden data released by the World Health Organization’s International Agency for Research on Cancer(IARC)in 2020 pointed out that the number of new liver cancer cases in China accounted for 45% of the global new cases and deaths when the number of new liver cancer deaths accounted for 47% of the global new deaths,respectively,which seriously threatened the lives and health of Chinese people.S1 P receptors S1PRs(including S1PR1,S1PR2,S1PR3,S1PR4 and S1PR5)on the cell membrane can be activated by S1 P,mediate multiple signaling pathways in cells,and regulate physiological functions of cells,such as cell proliferation,migration,apoptosis,angiogenesis,etc.These receptors are transmembrane proteins that can be coupled to different types of G proteins within the cell,activating downstream signaling molecules and transmitting signals.Tumor cells have the property of unlimited proliferation and metastasis.Studies have reported that S1PR2 can inhibit the migration of endothelial cells and melanoma cells,and can also inhibit the production of diffuse B-cell lymphoma,which may be a receptor with cancer suppressive effects.There have also been reports of S1PR2 as a pro-cancer receptor,and co-transfection of S1PR2 and S1PR3 in hepatoma cells and Jurkat cells resulted in S1P-mediated inhibition of cell proliferation and apoptosis.Therefore,through studying the role of S1PR2 in hepatocellular carcinoma and evaluating its value as a prognostic indicator and intervention target of hepatocellular carcinoma,personalized treatment can be better realized in the treatment of liver cancer,and may bring more clinical application value.However,at present,there are few reports on the role of S1PR2 in liver cancer at home and abroad,and the relevant reports have not explicitly elucidated the influence of S1PR2 on the biological behavior of hepatoma cells.Objective: The effect of S1PR2 on the biobehavior of HepG2 hepatoma cell lines was determined by in vitro experiments.The effect of S1PR2 on liver cancer was verified by in vivo experiments.The experimental results provide a theoretical basis for the therapeutic target of liver cancer.Methods: 1.Construct a human S1PR2 lentiviral expression vector,infect hepatoma cells HepG2,and establish a HepG2 cell line that stably expresses S1PR2;MTT detected the impact of S1PR2 on the proliferation of HepG2 cells,while scratch experiment revealed the effect of S1PR2 on the migration of HepG2 cells,cell cloning formation experiments revealed the influence of S1PR2 on HepG2 cell proliferation and invasion,flow cytometry revealed its effect on apoptosis,and the effect of S1PR2 on the biobehavior of hepatoma cells was clarified through the above experiments.2.The effect of S1PR2 on liver cancer was verified by using an animal model of subcutaneous tumor in nude mouse HepG2 cells.Results:1.S1PR2 lentivirus infection HepG2 cells were successfully constructed,which significantly increased the expression level of S1PR2 in hepatoma cells HepG2.2.The results of MTT experiments and cell cloning experiments showed that S1PR2 could inhibit the proliferation of HepG2 cells,scratch experiments confirmed that S1PR2 inhibited the migration ability of HepG2 cells,Transwell experiments confirmed that S1PR2 inhibited the invasion ability of HepG2 cells,and apoptosis experiments confirmed that S1PR2 could promote apoptosis of HepG2 cells.3.A tumor model of liver cancer in nude mice was successfully constructed to evaluate the effect of S1PR2,and the results showed that S1PR2 could inhibit the volume and weight of tumors formed subcutaneously in nude mice.Conclusion: S1PR2 can inhibit the proliferation,migration,invasion ability and apoptosis of HepG2 cells.S1PR2 inhibits tumor volume and weight formed subcutaneously in nude mice.The experimental results provide a theoretical basis for the therapeutic target of liver cancer.