Protective Effect Of MSCs Transplantation On Periventricular Leukomalacia In Neonatal Rats And Expression Of BDNF

Objective: To select a better animal model of leukomalacia in preterm infants by comparing different methods.And BDNF as the target expression factor,to explore the protective effect of mesenchymal stem cells on neonatal rat periventricular leukomalacia.Methods: To establish the animal model of leukomalacia of preterm infants by comparing the pathological changes and cerebral ischemic area of the neonatal rat model of lipopolysaccharide(infection),ischemia and hypoxia and the combined model of lipopolysaccharide and ischemia and hypoxia.MSCs were transplanted into the lateral ventricle at 24 h and 72 h after the establishment of the white matter injury model of premature infants.BDNF was used as the target expression factor.The expression of BDNF in the brain tissue of neonatal rats with leukomalacia was studied by immunohistochemistry and Western blots.Results: Different methods for modeling found lipopolysaccharide+ ischemia hypoxia combined model group of ischemic area is larger,the total area of brain slices 20.06±1.85%(P< 0.05),and modeling methods to detect visible modeling in 7 days after different pathology appeared different degree of white matter osteoporosis,and nerve fibers arranged neatly,for 21 days,the joint group typically seen disordered arrangement of nerve fibers,the model of all samples with coagulation necrosis and cystic cavity formation(P< 0.05);Softening can result in newborn rat cerebral white matter in the brain reduce BDNF expression,between the lateral ventricle mesenchymal stem cells transplantation can increase the expression of BDNF,24 htransplantation group,number of positive cells was 68.6±5.5 / each field of vision,increased more significantly than 72 h transplantation vision 48.3±4.1 / each field of vision(P < 0.01).Conclusion: By lipopolysaccharide + ischemia hypoxia group joint comprehensive the premature infant brain damage mechanical factors,the establishment of a more accord with clinical animal model;Ectomesenchymal stem cells to repair the newborn rat white matter in the softening process,24 h between mesenchymal stem cell transplant is more advantageous to the secretion of BDNF and repair of brain damage.