| Objective:To investigate the significance of serum ubiquitin carboxy-terminal hydrolase 1(UCHL1)and serum ubiquitin carboxy-terminal hydrolase 3(UCHL3)in evaluating the function of testis spermatocyte in patients with undescended testis.Methods:Serum UCHL1 and UCHL3 levels were detected in 49 patients with cryptorchidism admitted to our hospital from December 2020 to December 2021,including 32 patients with unilateral cryptorchidism(20 patients with right cryptorchidism,12 patients with left cryptorchidism)and 17 patients with bilateral cryptorchidism.The mean age was 75.28(50.32,96.01)months,including 7 cases ≤2 years old and 42 cases > 2years old.Another 50 normal male children were randomly selected as the control group,with an average age of 76.51(51.67,109.77)months,including 10 children ≤2 years old and 40 children > 2 years old.Serum concentrations of UCHL1,UCHL3 and related parameters of germ cell development were measured before and 6 months after operation.The differences between the two markers in serum were analyzed and compared among the groups and before and after surgery.Results:The data showed that there was no statistical difference in age and BMI between the cryptorchidism group and the control group.The volume of testis in the undescended testis group was significantly lower than that in the control group,and the other parameters related to external genitalia had no statistical significance.There was no significant difference in serum sex hormone levels.The serum UCHL1 and UCHL3 levels of the control group and the cryptorchtestis group were 14.98±2.60 before surgery and 14.32±2.21 6 months after surgery.The levels of UCHL1 in the cryptorchtestis group were higher than those in the control group(8.57±1.40 before surgery and 9.16±1.32 after surgery,respectively)(t=-19.576,P<grou0.001)and(t=-17.824,P<0.001),the difference was statistically significant.The difference level of UCHL1 before and after operation(Z=2.921,P=0.003)in cryptorchidism group was different from that in control group,and the difference was statistically significant.The UCHL3 level of the undescended testis group was12.22(10.84,13.20)before surgery and 12.53(11.33,13.41)6 months after surgery,which was lower than that of the normal control group(14.50(13.36,15.36)before surgery and14.05(13.04,15.54)after surgery.(Z=-5.863,P<0.001)(Z=-4.798,P<0.001),the difference was statistically significant.No difference was found in the UCHL3 data of the cryptorchidism group before and after operation.The level of serum UCHL1 and UCHL3 in unilateral cryptorchidism group was 15.09±2.55 higher than that in unilateral cryptorchidism group at 6 months after operation(t=-3.702,P<0.001),the difference was statistically significant.Preoperative UCHL3 level in bilateral undescended testis group was 11.33(10.33,12.80)lower than that in unilateral group(12.66(10.97,13.28)(Z=2.369,P=0.018),and the difference was statistically significant.Comparing the two indicators at different ages,there was no difference in serum UCHL1 level between patients less than 2 years old and those older than 2 years old,while there was no significant difference in serum UCHL1 and UCHL3 between patients less than 2years old and those older than 2 years old in healthy control group.Conclusion:The assessment of serum UCHL1 and 3 levels is helpful to understand the situation of germ cell apoptosis in children with undescended testis,and further clarify the change of testicular function in patients with undescended testis,so as to evaluate the recovery of patients with undescended testis after surgery,which may be used as a new indicator to evaluate the testicular function in patients with undescended testis in the future. |
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