Animal Model Experiment Of Prophyromonas Gingivalis Promoted The Progression Of Oral Squamous Cell Carcinoma By Activating The Axis Of CXCL2/CXCR2

Objective: To investigate the effect of the Porphyromonas gingivalis(P.gingivalis)promoting the oral squamous cell carcinoma(OSCC)progress through the chemokine c-x-c motif ligand 2 / chemokine receptor type 2(CXCL2 / CXCR2)axis in the oral cancer animal model.Methods: The SCC7 murine squamous cell carcinoma cell line was cultured in vitro.The cell and bacteria co-culture model was established using SCC7 and P.gingivalis.The tumor-bearing model was established in C57BL/6 mice.Different concentraitions of CXCL2/CXCR2 axis inhibitor SCH527123 were used for intervention.The animals were divided into four groups as follows: control group,high dose group,low dose group and experimental group respectively.The mouse tumor model was studied by observing the tumor volume,immunohistochemistry and enzyme-linked immunosorbent assay(Elisa).The expression of Gαi、P.gingivalis,CXCL2,CD66 b +,N-Cadherin in each groups was tested by immunohistochemistry and CXCL2 levels were tested by Elisa.Results: Tumor volume was significantly larger in experimental group than that in control group(P<0.01).When useing CXCL2 / CXCR2 axis inhibitors,compared with the experimental group,the tumor volume in low dose group decreased(P<0.05)and the tumor volume in high dose group decreased more significantly(P<0.01).The expression of Gαi in the experimental group was stronger than that in the control group,high dose group and low dose group(P<0.05).The difference between the low dose group and the high dose group was statistically significant,indicating that CXCL2/CXCR2 signal axis inhibitor SCH527123 successfully inhibited the signal axis.P.gingivalis,CXCL2,CD66 b +,N-Cadherin were expressed strong positively or positively in experimental group,negatively in control group,negatively or weakly in high and low dose groups.E-Cadherin was expressed strong positively,moderate positively,weak positively,negatively in control group,high dose group,low dose group and experimental group respectively.CXCL2 levels were highest in experimental group,which is statistically significant compared with the control group high dose group and low dose group(P<0.001).Conclusion: P.gingivalis promotes tumor progression by up-regulating the expression of CXCL2.When SCH527123 is used for intervention,tumor progression is inhibited.