Effect Of Cluster Needling At Scalp Acupoints Combined With Red Light On Visual Function And Expression Of BDNF And TrkB In Visual Cortex In Monocular Deprivation Amblyopia Rats

Objective:To investigate the effects of cluster needling at scalp acupoints combination with red light on visual function and expression of BDNF and TrkB in the visual cortex of monocular deprivation amblyopia rats,in order to find a new therapies for amblyopia and to establish a solid theoretical basis for the treatment of amblyopia with cluster needling at scalp acupoints and red light.Methods:The 30 clean and healthy Sprague Dawley(SD)newborns rats,male or female,were randomly selected and divided into five groups:normal group,control group,red-light group,Acupuncture group,Acupuncture group+red light group(hereinafter referred to as the combination group),each group has 6 rats.At 13 days after birth(P13),the left eyelid of newborn rats was sutured to establish a monocular deprivation amblyopia model.Among them,the normal group received no treatment,the model group had no intervention after successful modeling,and the red light group,acupuncture group,and combination group began on the second days after opening the eyelid after 2 weeks of suturing with red light,cluster needling at scalp acupoints,and cluster needling at scalp acupoints combined with red light treatment.Each group was evaluated visual acuity with the front claw touchdown reflex test and visual depth perception with visual cliff at 28 and 44 days after birth.The morphological structure of area 17 of the right visual cortex was observed by hematoxylin-eosin staining(hereinafter referred to as HE staining)at 4 2 days after birth.The chemistry of immunohistochemistry is used to detect the expression of BDNF and TrkB in the right visual cortex.To clarify that the cluster needling at scalp acupoints and red light may promote the improvement of visual function by raising the expression of BDNF and TrkB in visual cortex of monocular deprivation amblyopia rats.Results:1.Normal group and model group F-VEP resultsCompared to the normal group,the P2 wave incubation period in the model group was significantly prolonged,the difference was statistically significant(P<0.01).the P2 wave amplitude was significantly reduced,the difference was statistically significant(P<0.01).The rat model of monocular amblyopia was successfully established by deprivation for 2 weeks on the 13th days after birth.2.Visual acuity assessmentEach group was compared between the groups at the postnatal 28 days(before treatment).The success rate of the front paw ground reflection test in the model group,the red light group,and the combination group was significantly lower than in the normal group,there was a statistical difference(P<0.01).At the postnatal 44 days(after treatment),the success rate of the front paws touchdown reflex test in the combination group was significantly higher than the model group(P<0.01).The red light group and the acupuncture group was higher than the model group(P>0.05),lower than the combination group and the normal group(P>0.05).At the postnatal 28 and 44 days,the forepaw touch reflex test showed significantly higher than before treatment in the success rate of the red light group,acupuncture group and combination group,and there was a statistical difference in comparison within the group(P>0.05).3.Visual depth perception assessmentEach group was compared between groups at the postnatal 28 days(before treatment).The visual cliff exploration index of the model group,red light group,acupuncture group and combination group were statistically lower and different compared to the normal group(P<0.0 1);At the postnatal 44 days(after treatment),the visual cliff exploration index of the normal group,red light group,acupuncture group and combination group were significantly higher than those of the model group(P<0.0 1).The combination group was higher than the red light group and the acupuncture group(P<0.05),there was no statistical difference between the red light group and the acupuncture group(P>0.05).Comparisons between the groups at the postnatal 28 and 44 days.The visual cliff exploration index of the red light group,the acupuncture group and the combination group has been significantly improved than before treatment,and showed statistical differences(P<0.01).4.primary visual cortex morphological structureAt the postnatal 44 days(after treatment),the neurons in the right primary visual cortex of rats in the normal group had normal structure,clear cell shape,and large neurons,a rich and abundant cytoplasm,along with a clear bulge,and the nucleus of the nucleus was blue,large and round.In the model group,the structure of neurons of the right primary visual cortex of the cohort was arranged unevenly,the cell outline was not clear,the number of neurons was small,the cytoplasm was small,and the protrusions became shorter or disappeared,the neurons were degenerated,the size of the nucleus was small,hardening,decay and rupture were visible,accompanied by the proliferation of glial cells.Compared to the model group,the structure,number and morphology of neurons in the right primary visual cortex were reduced in the red light group and in the acupuncture group.The structure,number and morphology of neurons in the right primary visual cortex of the group gradually returned to normal.5.The expression of BDNF and TrkB in visual cortexAt the postnatal 44 days(after treatment),compared with the model group,the BDNF、TrkB expression was increased in the red light group,acupuncture group,the combination group and the normal group,and there were significant differences(P<0.05).The combination group was higher than the red light group and the acupuncture group(P<0.05),there was no statistical difference between the red light group and the acupuncture group,between the combination group and the normal group(P>0.0 5).Conclusion:1.F-VEP can be used to detect the success of monocular deprivation amblyopia model.2.The cluster needling at scalp acupoints and red light can improve the visual function of monocular deprivation amblyopia rats,including visual acuity and visual depth perception,and the combination effect is better.3.The cluster needling at scalp acupoints and red light can promote the morphological structure of the primary visual cortex in monocular deprivation amblyopia rats,and the combination effect is better.4.The cluster needling at scalp acupoints and red light upregulation of BDNF and TrkB expression in the visual cortex of monocular deprivation in amblyopia rats,and the combination effect is better.5.The cluster needling at scalp acupoints and red light may improve the visual function of monocular deprivation in amblyopia rats,and its mechanism of action may be related to upregulation of BDNF and TrkB expression in the visual cortex.