Preliminary Exploration Of The Molecular Mechanism Of EEF1A1 In Regulating Neuroinflammatory Processes In Parkinson’s Disease

Background: Studies have reported that Eukaryotic Elongation Factor 1A1(EEF1A1)may have an important role in neurodegenerative diseases.This study aims to investigate the molecular mechanism of EEF1A1 in the neuroinflammatory process of Parkinson`s disease(PD).Methods: The expression of EEF1A1 in U251 cells was inhibited by si RNA transfection.Then,RNA-seq sequencing was used to determine the Differentially Expressed Genes(DEGs)resulting from the EEF1A1 knockdown.Additionally,GO and KEGG enrichment analyses were performed to find the biological processes and signaling pathways engaged in the DEGs,as well as to screen for genes associated with neuroinflammatory processes that influence the development of PD.Further,we performed RT-q PCR to validate and initially explore the molecular mechanism of EEF1A1 in regulating neuroinflammatory processes in PD.Results:RNA-seq analysis revealed that EEF1A1 knockdown significantly up-regulated the expression of genes IL-6,GDF15,STC1,MT1 E,GPNMB,CCL5,MT1 X,A2M,and VIP at the transcriptional level in U251 cells.The GO and KEGG enrichment analysis revealed that nine genes with significantly elevated expression levels were enriched in a variety of inflammation-related signaling pathways such as tumor necrosis factor signaling pathway,humoral immune response,and inflammatory response.Conclusions:After EEF1A1 knockdown,significantly up-regulated genes GDF15,STC1,MT1 E,MT1X,GPNMB,VIP,and A2 M are involved in delaying the neuroinflammatory process of PD,while genes IL-6 and CCL5 are involved in exacerbating the neuroinflammatory process,suggesting that EEF1A1 may be involved in the development of PD at the transcriptional level by regulating the expression of genes related with the neuroinflammatory process of PD.