Characterization And Biocompatibility Study Of GO/HA Coating Materials On Titanium Substrates Prepared By Electrochemical Deposition

Objective:To study the biocompatibility of GO/HA coatings on mouse BMSCs and the osteogenic induction effect.Methods:By electrochemical deposition method in the basement of Ti and the preparation of HA coating on the surface of GO/HA composite coating.The surface morphology,phase structure,functional groups,element composition and surface hydrophilicity of the coating were analyzed by SEM,EDS,XPS,XRD,Ramman spectrometer and contact Angle measuring instrument.BMSCs of Balb/c mice were cultured in vitro and then identified by flow.GO/HA composite coating material was co-cultured with BMSCs.The experimental groups were:GO/HA-Ti in the experimental group,HA-Ti in the control group and Ti in the blank group.The proliferation,morphology and growth status of coated surface cells were evaluated by CCK-8 cell counting,DAPI staining and SEM.Alizarin red-S calcium nodule staining,ALP activity staining and real-time PCR were used to investigate the effects of each coating on osteogenic differentiation of BMSCs.Results:①Both HA and GO/HA coatings can be prepared on titanium surface by electrochemical deposition.SEM and EDS results show that the GO/HA coatings are more flat,compact and uniform than HA coatings and pure Ti;②The results of XPS,XRD and Raman spectra show that HA and GO/HA coatings are successfully prepared on the surface of Ti;③The results of contact Angle showed that the hydrophilicity of GO/HA coating was better than that of HA coating and pure Ti,and the difference was statistically significant(P<0.05);④Compared with the control group and blank group,the experimental group had the strongest ability to promote cell proliferation,and the cell morphology and extension were better,the difference was statistically significant(P<0.05);⑤Alizarin red-S and ALP staining showed that the experimental group had more red calcium nodules,and the blue was deeper and wider than the control group and the blank group;⑥Real-time PCR results showed that the expression of bone promoting genes in each group showed an increasing trend over time.However,the expression level of promotor bone gene in the experimental group was significantly higher than that in the control group and blank group within the same culture time,and the difference was statistically significant(P<0.05).Conclusion:GO/HA coating material with excellent physicochemical properties was successfully prepared by electrodeposition.GO enhances the biocompatibility of the materials to a certain extent,and promotes the osteogenic differentiation of BMSCs to a certain extent.