Effects Of Acteoside On The Proliferation And Osteogenic Of MC3T3-E1

Objective:To investigate the effect of acteoside on the proliferation and osteogenic differentiation of MC3T3-E1 cells.Methods:Prepare piloside solution with a concentration of 1 mmol/L-10^-7mmol/L.After 1、2、3 and 5 days of treatment,the cell proliferation rate and cytotoxic effects of the cells each group was detected by CCK-8assay.After 7days and 14days,alkaline phosphatase（ALP）staining and quantitative detection of ALP activity and early osteogenesis capacity in MC3T3-E1 cells.After 14days and 21days,alzarin red staining and semi-quantitative detection of the number of calcium nodules and late osteogenesis capacity in MC3T3.The m RNA expression levels of ALP、type-I collagen（COL-I）、Runt-associated transcription factor2（RUNX2）、Osteocalcin（OCN）and Osterix were detected were detected using real-time Polymerase Chain Reaction.Results:The result of CCK-8 showed that 10^-3,10^-4,and10^-5mmol/L acteoside significantly promoted cell proliferationamong（P<0.001）.The acteodide concentration of 10^-4、10^-5mmol/L significantly promoted the ALP activity,and promote early osteogenesis in MC3T3-E1 cells.The group of 10^-5mmol/L acteoside significantly increase the number of calcium nodules stained with alizarin red.Treatment of MC3T3-E1 cells with 10^-3,10^-4,10^-5mmol/L acteoside significantly up-grade the m RNA expression levels of Col-1,RUNX 2,OCN,and Osterix.Conclusion:The concentration of 10^-4、10^-5mmol/L acteoside can promote the proliferation and osteogenic differentiation of MC3T3-E1 cells.