| Objective: Type 2 diabetes(Diabetes mellitus type 2,T2DM)is one of the non-infectious endocrine metabolic-related diseases that seriously threaten human health today,and T2 DM is considered an independent risk factor for cardiovascular disease.The International Diabetes Federation estimates that the number of people with T2 DM will reach 700 million worldwide by 2045 and will become the greatest threat to human health and safety in the 21 st century.This study investigated the association between endolipin gene loci rs61330082 and rs4730153 single nucleotide polymorphisms and endolipin levels with T2 DM risk in Xinjiang population from a genetic-epidemiological perspective.METHODS: A case-control study design was used to select 1256 study subjects,with the T2 DM group containing 551 diabetic patients and the control group containing 705non-diabetic people.the T2 DM and control groups were matched in terms of sex and age.The study population was recruited from T2 DM patients hospitalized with coronary heart disease as a diagnosis in the Department of Cardiovascular Medicine of the First Affiliated Hospital of Xinjiang Medical University from January 2016 to December 2019,and those without diabetes mellitus hospitalized with coronary heart disease as a diagnosis in the Department of Cardiovascular Medicine of the First Affiliated Hospital of Xinjiang Medical University during the same period.The SNPscan TM high-throughput single nucleotide polymorphism genotyping method was used to genotype the endolipin locus rs61330082 and rs4730153,and the serum endolipin levels were measured by ELISA and analyzed using the SPSS25 software package.Unconditional binary logistic regression analysis was applied to determine the association between endolipin gene loci rs61330082 and rs4730153 polymorphisms and endolipin levels with the risk of T2 DM by excluding the confounding factors.RESULTS: The genotype distributions of both T2 DM and control groups were(AA,AG,GG)type,and the differences in the dominant model,allelic genotype,and recessive model gene distributions of the endolipin locus rs4730153 were statistically significant(P < 0.05)when compared.In the T2 DM group,the frequency of the G allele of the mutation in the endolipin locus rs4730153 and the frequency of the GG genotype of the recessive model mutation were higher than those in the control group,and the differences were statistically significant(P<0.05),while the frequency of the dominant model and allele genotype distribution of the endolipin locus rs61330082 genotype were not statistically significant in the two groups(P>0.05),and the endolipin The frequency of distribution of AA+AG genotypes in the invisible model of rs61330082 was higher in the control group than in the T2 DM group,and the difference was statistically significant(P < 0.05).Serum endolipin levels in T2 DM patients were higher than those in the control group,and the difference was statistically significant(P<0.05).Using unconditional binary logistic regression analysis to adjust for confounders of T2 DM,it was concluded that hypertension,hypertriglyceridemia,hypercholesterolemia,low HDL cholesterolemia,GG genotype with rs4730153 mutation in the endolipin locus,and increased serum endolipin levels were risk factors for T2 DM,and compared with people with normal blood pressure,hypertensive patients had a The risk was increased0.143-fold in hypertensive patients compared to those with normal blood pressure(OR=1.43,95% CI1.09-1.88,P<0.05).The risk of diabetes was 0.157 times higher in patients with hypertriglyceridemia compared to those with normal triglycerides(OR=1.57,95% CI1.14-1.218,P<0.05).Patients with hypercholesterolemia had a 0.569-fold higher risk of diabetes than those with normal cholesterol levels(OR=5.69,95% CI3.35-9.66,P<0.05).The risk of diabetes was 0.190 times higher in patients with low HDL cholesterol than in those with normal HDL cholesterol(OR=1.90,95% CI1.41-2.55,P<0.05),and the risk of diabetes was 0.104 times higher in those with increased white blood cell count compared to those with normal white blood cell count(OR=1.04,95% CI1.01-1.08,P<0.05).1.08,P<0.05).The risk of diabetes was 0.120-fold higher in those with increased serum endolipin levels compared to those with normal serum endolipin levels(OR=1.20,95% CI1.17-1.22,P<0.05).The risk of diabetes was 0.241-fold higher in those with the rs4730153 GG genotype of the endolipin locus compared to those with the AG+GG genotype(OR=2.41,95% CI1.10-5.32,P < 0.05).The area under the ROC curve to evaluate the diagnostic value of serum endolipin levels for type 2 diabetes was 0.845(95%CI: 0.822-0.868)The Cuttoff value to predict the probability of type 2 diabetes was36.09% with a sensitivity of 75.7% and specificity of 86.4%.The GG genotype with rs4730153 mutation in the endolipin locus for the diagnosis of type 2 diabetes was evaluated with an area under the ROC curve of 0.668(95% CI: 0.636-0.700)with a Cuttoff value of 20.04% and a sensitivity of 60.1% for predicting the probability of type 2diabetes.The specificity was 66.1%.CONCLUSION: The GG genotype of the endolipin locus rs4730153 and increased serum endolipin levels are associated with the risk of developing T2 DM.Increased serum endolipin levels and the GG genotype with mutations at the endolipin gene rs4730153 locus are risk factors for T2 DM.It can be used as a biological marker to predict type 2 diabetes.The GG genotype with mutations at the endolipin locus rs4730153 may be a candidate gene for predicting phenotypic changes in the development of T2 DM,whereas the endolipin locus rs61330082 polymorphism was not associated with the risk of developing T2DM... |
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