Effect And Significance Of Bevacizumab On H1299 Cell Function Experiment And Transcriptomics In Human Lung Cancer

Part Ⅰ In vitro study of the effects of bevacizumab on human lung cancer H1299 cell proliferation,migration invasion and apoptosisObjective To study the direct effect of bevacizumab on the antitumor effect of human lung cancer H1299 cells in vitro,and to preliminarily explore its possible mechanism.Methods H1299 cells were treated with different concentrations of bevacizumab(0.1μg/ml,1μg/ml,10μg/ml,100μg/ml),and cell counting kit 8(CCK-8)was used to detect the proliferation ability of H1299 cells.Further analysis of cell migration and invasion capacity by scratch assay and Transwell chamber method.Flower cytometry was used to examine its effect on apoptosis.Results CCK-8 experiments showed that compared with the blank control group,bevacizumab had a bidirectional effect on the proliferation ability of H1299 cells.Bevacizumab had the strongest inhibitory ability on cells at a drug concentration of 10μg/ml(P<0.05),and a high concentration of 100μg/ml bevacizumab promoted the proliferation of H1299 cells(P<0.05).The scratch experiment showed that when the drug acted for 24 hours,the migration distance after10μg/ml bevacizumab treated the cells became significantly shorter,and the migration ability decreased significantly(P<0.05).The results of Transwell experiments showed that the cell migration and invasion ability decreased significantly compared with the control group when the drug concentration was10μg/ml,and the inhibitory ability decreased with the increase of drug concentration at 100μg/ml bevacizumab(P<0.001).The results of apoptosis detection showed that bevacizumab was concentration-dependent on apoptosis,and the ability to promote apoptosis became stronger with the increase of drug concentration.Conclusion Bevacizumab only inhibits the proliferation and migration of H1299 cells within a certain concentration range,but presents a concentration-dependent effect on apoptosis.Part Ⅱ Effect and significance of high concentration of bevacizumab on transcriptomics of human non-small cell lung cancer cellsObjective To investigate the effect of high concentrations of bevacizumab on the transcriptomics of human non-small cell lung cancer cell line H1299.Methods After 72 hours of treatment with 100μg/ml bevacizumab cells,cells without drug intervention were used as the control group,and gene transcription capacity,gene ontology(GO)function,Kyoto gene and basic group database(KEGG)signaling pathway,and disease ontology(DO)function were analyzed.Results A total of 1145 differentially expressed genes were screened out by gene difference analysis,of which 704 were up-regulated and 441 were down-regulated.The top 10 upregulated genes were NΜPR1,SOX6,RP1-309F20.4,PAPPA2,ZMYND12,RP11-219D15.3,GOLGA8 O,OR1F1,AS3 MT,KCNIP2-AS1.The top 10 down-regulated genes were OXCT1,THBD,TGM4,TREX2,ATOH1,EXD1,RΜNX1T1,KCNS1,DLK1,RP11-573D15.3.The results of functional analysis showed that high concentrations of bevacizumab could change the transcription ability of some genes in H1299 cells,and participate in the regulation of GO function,KEGG signaling pathway and DO function.Conclusion High concentration of bevacizumab has a certain effect on the transcriptomics of H1299 cells.