The Role Of Serum Lectin Mannose-binding 2 In The Diagnosis Of Sepsis And Its Prognostic Value

Objective To study the role of serum LMAN2 in the diagnosis of sepsis and its prognostic value.Methods LMAN2 protein was screened by quantitative proteomic detection of serum DIA.Serum LMAN2 was measured by Enzyme linked immunosorbent assay（ELISA）in 20 age-and gender-matched healthy controls and 109 sepsis patients within 24 hours after their admission to ICU.We also collected clinical and laboratory variables.Vital signs（blood pressure,heart rate,respiratory rate,and body temperature）,routine laboratory test results（creatinine,bilirubin,platelet count,C-reactive protein（CRP）,Procalcitonin（PCT）,hemoglobin,hematocrit,sodium,potassium,white blood cell count and blood culture）,blood gas analysis（p H,lactate（Lac）,PaO₂,PaCO₂,bicarbonate（HCO3）,and base excess（BE））,Sequential Organ Failure Assessment（SOFA）score,Acute Physiology and Chronic Health Evaluation（APACHE Ⅱ）Ⅱ score and personal information（age,sex）were collected.Results On the day of entering ICU,serum LMAN2 level in septic patients were higher than that of healthy control group,and the serum LMAN2 level of septic shock patients was significantly higher than that of septic group.Serum LMAN2 level in non-survival group was significantly higher than that in survival group（P<0.001）.Serum LMAN2 level on the day of admission to ICU was associated with 28-day mortality in septic patients.COX regression analysis showed that serum LMAN2 level（>1.28ng/ml）in the high expression group was an independent risk factor for 28-day mortality in patients with sepsis.Conclusion This study showed that serum LMAN2 level can predict the occurrence of sepsis and is significantly correlated with mortality,and serum LMAN2 level can be used as an independent prognostic indicator of sepsis patients.