Changes Of Intestinal Flora And Serum Metabolites In Patients With Traumatic Brain Injury

Background: Environmental factors have a very important impact on the outcome of patients with traumatic brain injury(TBI).However,the relationship between intestinal microbiome,serum metabolome and environmental factors in TBI patients is still unclear.Therefore,we compared the intestinal microbiome and serum metabolome of TBI patients and healthy individuals to analyze the differences of specific bacteria and serum metabolism.Methods: We collected patient data from 2019 to 2020 for research.The stool and serum samples of TBI patients were collected immediately after admission,and the healthy control group samples were collected for comparison.Results: From October 2019 to June 2020,a total of 47 TBI patients were enrolled.According to the Glasgow Coma Scale(GCS)at the time of admission,they were divided into three TBI groups(G1: 3-8,G2: 9-12 and G3: 13-15).The age,gender and other factors of the patients were similar.We found that the microbial diversity and abundance index(Ace and Chao)were significantly different between the healthy control group and TBI group.There are significant differences in microbial diversity and species richness index(Ace and Chao)among G1,G2 and G3 TBI groups.The serum metabolic phenotype of TBI group was significantly different from that of healthy control group.Conclusion: Lipid-related metabolic pathway(synthesis and degradation of ketone body)is the most abundant in TBI group,which provides theoretical basis for TBI patients to adopt ketogenic diet.