| Objective : IS a kind of neurosystemic disease with high morbidity,disability and mortality.In recent years,the trend of younger onset is becoming more and more serious.Previous studies have suggested that AS IS closely related to abnormal metabolism in the body,and early-onset AS is an important cause of IS in young people.At present,abnormal metabolites in the serum of such patients are not very clear.In this study,metabolomics analysis was performed on the serum of patients with earlyonset AS-type IS to screen serum metabolites associated with the onset of early-onset AS-type IS.Methods: Using ultra-high performance liquid chromatography(HPLC)-Q-TOF MS non-targeted metabolomics analysis technique,the differential expression metabolites in serum of 40 patients with early-onset AS-type IS,30 elderly patients with AS-type IS and 30 control patients were quantitatively analyzed,and the signature metabolites closely related to disease occurrence were screened out.Results: In the young IS group,there were 35 males and 5 females,aged(39.43±5.29)years.There were 19 males and 11 females in the middle-aged and elderly IS group,aged(67.23±11.06)years,and the age difference between the two groups was statistically significant(P<0.05).There were 21 males and 9 females in HC group,aged(40.66±3.99)years,and there was no significant difference in age and gender between HC group and young AS-type IS group(P>0.05).The proportion of smoking,drinking,hypertension and diabetes in the young IS group was higher than that in the old group,but the difference was not statistically significant(P>0.05).There were no significant differences in serum total cholesterol and low density lipoprotein between the young IS group and the middle-aged and elderly IS group(P>0.05).The proportion of males and the proportion of hyperuricemia in the young IS group was significantly higher than that in the middle-aged and elderly group,with statistical significance(P<0.05).The contents of blood glucose,triglyceride,glycosylated hemoglobin and homocysteine in youth group were higher than those in middle-aged and elderly group,while high density lipoprotein was lower than those in middle-aged and elderly group,the difference was statistically significant(P<0.05).The levels and composition of APTT,INR and bilirubin were significantly different between the two groups(P<0.05).There was no significant difference in MAP,NLR,PLR and white sphere ratio between the two groups(P>0.05).Metabolomics analysis showed that there were 192 different metabolites in serum between the young IS group and HC group.Compared with the young control group,66 metabolites increased and 126 metabolites decreased,the difference was statistically significant.There were 207 different metabolites in serum between the young IS group and the middle-aged and old IS group,among which 138 metabolites were significantly increased and 69 metabolites were significantly decreased,the difference was statistically significant.These serum differential metabolites are involved in several pathways such as glucose metabolism,lipid metabolism,amino acid metabolism and nucleotide metabolism.KEGG pathway and enrichment analysis of different metabolites between the young IS group and HC group showed that the differences in Taste transduction,Neuroactive ligand-receptor interaction and Purine metabolism and other 20 important pathways showed significant changes;KEGG pathway and enrichment analysis of different metabolites between the young IS group and the middle and old IS group showed that the Central carbon metabolism in cancer,Protein digestion and absorption,ABC Significant changes were observed in 20 important pathways such as transporters.Conclusions: The results of this study showed that the serum metabolites of young AStype IS patients were different from those of middle-aged and elderly AS-type IS patients and HC group,with changes in glucose metabolism,lipid metabolism,amino acid metabolism and nucleotide metabolism.The changes of some serum metabolites may accelerate the progression of AS,which can be used AS a risk factor or protective factor for early-onset AS type IS,so as to facilitate clinical monitoring and estimate the risk of early-onset AS.However,because this experiment was a cross-sectional study,part of the serum differential metabolites may be the result of the production of earlyonset AS. |
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