Correlation Of MTHFR C677T Gene Polymorphism And Hcy With Renal Damage In Patients With H-type Hypertension

Objective:To investigate the correlation of MTHFR C677T gene polymorphism and Hcy with renal damage in patients with H-type hypertension,in order to provide a basis for early clinical prevention and precise individualized treatment to reduce the occurrence of renal damage and improve the prognosis of patients with H-type hypertension.Methods:A total of 291 hypertensive patients hospitalized in the Department of General Medicine of the Affiliated Hospital of Chengde Medical College from September 2020 to March 2021 were selected,and another 60 health check-ups at our hospital during the same period were selected as the control group.The hypertensive patients were divided into H-type hypertension group(Hcy≥10μmol/L)and non-H-type hypertension group(Hcy<10μmol/L)according to Hcy levels.The general data of the patients were collected and their characteristics were observed.The patients’Fasting blood glucose,blood lipids,renal function indexes,plasma Hcy and other biochemical indexes,genotyping,and e GFR were calculated using the CKD-EPI formula.general data and biochemical indexes,genotype frequencies,Hcy levels between different genotypes were compared between the three groups,and factors influencing renal damage among patients with type H hypertension were analyzed.Quantitative data in this paper were described using(X±S)if they conformed to a normal distribution,independent samples t-test for comparison between two groups,ANOVA for comparison between multiple groups,and LSD-t-test for two comparisons between multiple groups.Qualitative data were described by number of cases and rate(%),and theX~2test was used for comparison between groups.Regression was performed using binary logistic regression and P<0.05 was considered a statistically significant difference.Results:1.The differences were not statistically significant(P>0.05)when comparing gender,age,duration of hypertension,history of smoking,history of alcohol consumption,BMI,FPG,BUN,TC,TG,HDL and LDL among the three groups.The systolic blood pressure,diastolic blood pressure,Hcy,UA,and Scr levels in the H-type hypertension group were higher than those in the non-H-type hypertension group and the control group,and the e GFR was lower than that in the non-H-type hypertension group and the control group,and the differences All differences were statistically significant(P<0.05).The levels of systolic blood pressure,diastolic blood pressure,UAand Scr were higher in the non-H hypertension group than in the control group(P<0.05),and the levels of e GFR were lower than in the control group(P<0.05),while the differences in Hcy were not statistically significant when compared with the control group(P>0.05),(see Table 1).2.The distribution of the MTHFR C677T gene in all three populations was consistent with the Hardy-Weinberg law of genetic equilibrium(P>0.05)and was representative of the population,(see Table 2).3.The MTHFR C677T genotype and allele frequencies were statistically different in the three groups(P<0.05).The TT genotype frequency(34.2%)was higher in the H hypertension group than in the non-H hypertension group(20.3%)and the control group(16.7%),and the T allele frequency(56.5%)was higher than in the non-H hypertension group(33.3%)and the control group(29.2%),all with statistically significant differences(P<0.05).the CT genotype frequency(44.6%)in the H hypertension group was was higher than that of the non-H hypertension group(26.1%)and the control group(25.0%),the CC genotype frequency(21.2%)was lower than that of the non-H hypertension group(53.6%)and the control group(58.3%),the C allele frequency(43.5%)was lower than that of the non-H hypertension group(66.7%)and the control group(70.8%),and the TT genotype frequency of the non-H hypertension group(20.3%)was higher than that of the control group(16.7%),and all differences were statistically significant(P<0.05),(see Table3).4.The comparison of Hcy levels among different genotypes in all three groups was statistically different(P<0.05),with all TT genotypes having significantly higher Hcy levels than CT genotypes and CC genotypes,and CT genotypes being higher than CC genotypes,(see Table 4).5.In patients with H-type hypertension,binary logistic regression(backward LR method)was performed with the occurrence of renal damage as the dependent variable and age,gender,duration of hypertension,history of smoking,history of alcohol consumption,SBP,DBP,BMI,FPG,blood lipids,UA,Hcy and MTHFR C677T genes as independent variables,and the results showed that SBP,UA,gender,Hcy,CT and TT genotypes were risk factors for the occurrence of renal damage in patients with H-type hypertension,(see Table 5).Conclusions:1.Mutations in the MTHFR C677T gene cause an increase in plasma Hcy levels.2.Elevated Hcy levels increase the risk of developing renal damage in a population with H-type hypertension.3.Mutations in the C to T of the MTHFR 677 locus are a risk factor for the development of renal damage in people with H-type hypertension,with CT and TT types being the susceptible genes.4.Emphasis should be placed on screening for MTHFR C677T gene polymorphisms in hypertensive populations,and monitoring of Hcy levels should be strengthened in those carrying the T allele at high risk,which is important for the early prevention and treatment of renal damage in H-type hypertension.