Correlation Between P16 Protein And Methylation Of PAX1 Gene And Cervical Cancer And Precancerous Lesions

Objective:The main objective of this project is to study P16 protein and the methylation of Paired box gene 1(PAX1)in different degrees of cervical precancerous lesions and cervical cancer cells,and focus on the analysis of both high-grade squamous intraepithelial lesions and cervical cancer between the two groups.And to evaluate the significance of their separate diagnosis and combined diagnosis in the diagnosis of different cervical lesions.Methods:Collect data:A total of 120 patients diagnosed with low-grade squamous intraepithelial lesions,high-grade squamous intraepithelial lesions and cervical squamous cell carcinoma admitted to our hospital from 2020 to2021 were selected as research objects,and were divided into cervical low-grade squamous intraepithelial lesions group(52 cases),cervical high-grade squamous intraepithelial lesions group(35 cases)and cervical cancer group(33 cases)according to pathological results.Cervical paraffin specimens were collected from each group.P16 protein:The expression of P16 protein in different cervical lesions was determined by immunohistochemical assay.PAX1 gene:The methylation of PAX1 gene was qualitatively analyzed by reverse transcription polymerase chain reaction(PCR).According to the experimental results,the methylation rate of PAX1 and the positive expression rate of P16 protein in each group were analyzed.Results:1.There was no statistical significance in age among the three groups(F=2.711,P=0.071>0.05),and there was no significant difference between age and expression of P16 and PAX1(χ2=0.077,P=0.781>0.05;χ2=3.219,P=0.073>0.05);2.Spearman correlation analysis showed that P16 and PAX1 were positively correlated with the degree of cervical lesions(P<0.05).3.The positive expression rates of P16 in LSIL,HSIL and SCC were21.15%,91.42%and 100.00%,respectively,with statistical significance(χ~2=70.840,P<0.05);4.The expression of P16 in the low grade and high grade groups was statistically significant(χ2=41.330,P<0.05);There was no significant difference between the two groups(χ~2=2.959,P=0.239>0.05).5.The positive expression rates of PAX1 were not all the same among the three groups.The positive expression rates of PAX1 were 13.46%,45.71%and 87.88%in LSIL,HSIL and SCC,respectively,with statistical significance(χ~2=45.644,P<0.05);6.The expression of PAX1 in the low grade and the high grade groups was statistically significant(χ~2=11.19,P=0.001<0.05);There was statistical significance in the expression of high grade cervical cancer and cervical cancer(χ~2=13.49,P<0.05);7.Compared with P16,PAX1,series and parallel for the differential diagnosis between low and high levels,P16 alone is the best diagnosis;8.Compared with P16,PAX1,series and parallel in the differential diagnosis between high-grade cervical cancer and cervical cancer,The single diagnosis and tandem diagnosis of PAX1 were statistically significant.However,the diagnostic efficacy of PAX1 alone and in tandem was not statistically significant by Delong test(P=0.3173>0.05).9.For P16,PAX1,series and parallel,series diagnosis is the best for determining whether cancer is diagnosed.Conclusions:1.The expression of P16 protein and methylation of PAX1 were positively correlated with the degree of cervical lesions,and all of them had certain guiding significance for the clinical diagnosis of cervical lesions;2.P16 alone has the most advantage in differentiating low-grade intraepithelial lesions from high-grade intraepithelial lesions;3.For the differentiation of high-grade squamous intraepithelial lesions and cervical cancer,the single diagnosis and tandem diagnosis of PAX1showed statistical significance,but there was no significant difference in the diagnostic efficacy of the two diagnostic methods for the differentiation of the two lesions;4.Tandem diagnosis is most advantageous in the differentiation between squamous intraepithelial lesions and cervical cancer.