SF/PLCL Cospining Inner Oriented Loaded AM80 Small Diameter Artificial Vascular Graft

With the continuous development of economic level and the general improvement of human living standard,cardiovascular diseases have become the most morbid and mortal diseases worldwide.Vascular occlusion due to atherosclerosis,inflammation,autoimmune and other causes require surgical reconstruction.Autologous vessels,allogeneic vessels,or artificial vessels are often used clinically for revascularization.Autologous vessels are derived from healthy vessels(such as internal mammary artery,saphenous vein,etc.)in their own sites,which are not rejected by the body,but their size and source are limited,often limited by the patient’s underlying physical condition,and can additionally increase secondary trauma to the body.Allogeneic vessels are in ample supply,but current technical means make it difficult to completely avoid rejection.In contrast,artificial blood vessels are abundantly available and easy to prepare,so it has become the most popular alternative in clinical practice.In recent years,large-diameter artificial vascular grafts prepared from materials such as expanded polytetrafluoroethylene or polyester have been widely used,while small-diameter artificial blood vessels less than 6 mm in diameter are still in the stage of development and exploration,and no small-diameter artificial blood vessel products have been marketed yet.Purpose: The aim of this study is to improve the structure and composition of small-diameter artificial blood vessels and prepare small-diameter artificial vascular grafts with good biocompatibility and mechanical properties,so that the artificial vascular grafts can achieve rapid endothelialization after transplantation into animals.At the same time,we hope that the components of the artificial vessels can inhibit the proliferation of synthetic smooth muscle cells that differentiate from the vascular mesoderm in response to injury.In the course of the study,the method to improve the long-term patency of small-diameter artificial vascular grafts was continuously explored in combination with cytological experiments and animal experiments.METHODS: Natural polymer materials were co-spun with synthetic polymer materials by electrostatic electrospinning technology,and nano-drug carriers were applied to load drugs that inhibit the proliferation of synthetic smooth muscle cells,and bilayer small caliber artificial vascular grafts with axially oriented inner layer were prepared.RESULTS: In this study,we took the clinical demand for small-diameter artificial vascular grafts as the starting point and prepared a functionalized PLCL/SF/MCM-41-AM80/OR small-diameter artificial blood vessel by co-spinning PLCL and SF as the main materials with AM80-loaded nanoporous silica.The long-term and stable slow release of AM80 by MCM-41 achieved the goal of regulating the excessive proliferation of smooth muscle cell layer without affecting the endothelialization process,thus achieving the two major goals of endothelialization and long-term patency of the implanted small bore artificial vessel.Conclusion: In this study,we designed and prepared small-diameter artificial vascular grafts that have undergone several experiments and tests.Statistical analysis of the experimental and test data showed that the parameters obtained basically met the expectations.It is expected that the results of the PLCL/SF/MCM-41-AM80/OR small-diameter artificial vessels in vivo tests in animals will further demonstrate the utility and potential for translational application of our designed small-diameter artificial vessels.