| Purpose:By observing the therapeutic effect of moxa combustion products combined with TDP physiotherapy instrument on rats with skin infection model,the possible mechanism of moxa combustion products combined with TDP physiotherapy instrument to improve wound healing in rats with skin infection was elaborated.Methods:Healthy male SD rats were randomly divided into 5 groups: normal group,model group,moxa combustion products treatment group,TDP physiotherapy treatment group and moxa combustion products combined with TDP physiotherapy treatment group,7rats in each group.Except for the normal group,0.2ml of the same concentration of Staphylococcus aureus solution was dripped into the back wounds of the rats in each group in turn,and the model was successfully replicated if purulent secretions were produced from the wounds.After successful replication,rats in the model group were treated with saline in the wound,rats in the moxa combustion product group were treated with moxa combustion product in the wound,rats in the TDP group were treated with TDP physiotherapy for 30 min,and rats in the combined treatment group were treated with moxa combustion product in the wound and TDP physiotherapy for 30 min,twice a day for 7 days.The changes in body weight and wounds of each group were recorded during the treatment period,and the morphological changes of their skin wounds were photographed before and on the 7th day of treatment,and the area of the wounds was calculated.The pathological changes of the wounds were observed by HE staining and the serum levels of Tumor necrosis factor alpha(TNF-α),interleukin-6(IL-6)and interleukin-10(IL-10)were measured by ELISA.And the levels of transforming growth factor beta 1(TGF-β1)and vascular endothelial growth factor(VEGF)in infected wound tissues were determined by immunohistochemistry to elucidate the possible mechanism of improving wound healing in a rat model of skin infection by combining moxa combustion products with TDP physiotherapy.Results:1.Wound healing in each group: the wound in the model group healed slowly,with purulent secretions still exuding from the wound;the wound in the remaining treatment groups healed significantly,and the wound area was significantly reduced compared with that in the model group(P<0.05).The combined treatment group was more effective,with a statistically significant reduction in wound area compared to the other two treatment groups(P<0.05).2.The results of HE staining of skin wounds: the model group still had a large number of neutrophils and other inflammatory cells infiltrating the wounds,while the inflammatory infiltrations in the wounds of the remaining treatment groups were significantly improved.3.Comparison of changes in the serum levels of TNF-α,IL-6 and IL-10 in rats:compared with the normal group,the serum levels of TNF-α and IL-6 in rats of the model group were increased and the levels of IL-10 were significantly decreased(P<0.01),which was statistically significant.After 7 days of treatment,compared with the model group,the serum levels of TNF-α and IL-6 in the rats of the moxa combustion products group,the TDP group and the combined treatment group were significantly lower and the levels of IL-10 were significantly higher(P<0.05),which was statistically significant.Among them,the levels of TNF-α and IL-6 in the serum of rats in the combined treatment group were significantly lower and the level of IL-10 was significantly higher compared with other treatment groups(P<0.05),which was statistically significant.4.The results of TGF-β1 and VEGF content measurement in rat traumatic tissues:After 7 days of treatment,TGF-β1 and VEGF produced in traumatic tissues of rats in the moxa combustion products group,TDP group and combined treatment group were significantly increased compared with the model group(P<0.05),which was statistically significant.Compared with the other treatment groups,the amount of TGF-β1 and VEGF in the traumatic tissues of rats in the combined treatment group was significantly increased(P<0.05),which was statistically significant.Conclusion:Treatment with moxa combustion products combined with TDP physiotherapy instrument significantly improved wound healing in skin-infected rats,and the mechanism may be related to changes in the levels of inflammatory factors TNF-α,IL-6,IL-10 and TGF-β1 and VEGF growth factors. |
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