| Objective: To explore the expression of syntaxin6(STX6)in epithelial ovarian cancer(EOC),and to analyze the effect of STX6 expression differences on the prognosis of patients.At the same time,the effect of STX6 expression regulation on the proliferation and migration of ovarian cancer cells were studied to provide new ideas for further understanding of the pathogenesis and development of ovarian cancer and exploration of therapeutic targets.Methods:(1)The Kaplan-Meier Plotter database was used to analyze the effects of STX6 expression differences on overall survival(OS)and progression free survival(PFS)in ovarian cancer patients.(2)Retrospectively analyzed the clinical data of EOC patients,and used immunohistochemical methods to detect the expression of STX6 in postoperative pathological paraffin samples of EOC patients;The relationship between STX6 expression differences and various clinicopathological indexes of patients was studied,and the correlation between STX6 expression and patient prognosis was analyzed.(3)Fresh samples of primary tumor tissue,peritoneal metastasis from EOC patients and normal ovarian tissue(ovarian resection due to benign disease)from health donors were collected,and the expression of STX6 was detected by q RT-PCR and immunoblotting methods.(4)In vitro cultured ovarian cancer cell line SKOV3,which performed with STX6 gene overexpression and knockdown by plasmid transfection and RNA interference technology,respectively.Then the expression level of STX6 in SKOV3 cells were verified by immunoprotein blotting,and then the cell viability,proliferation and migration ability of SKOV3 cells were detectd by CCK-8 experiment,clonal proliferation experiment and cell scratch experiment,respectivly.Results:(1)The analysis of Kaplan-Meier Plotter database found that there were significant differences in OS and PFS between ovarian cancer cases with high and low expression of STX6,and both the OS and PFS of patients with high expression of STX6 were significantly reduced compared with those in the low expression group of STX6(P<0.05).(2)The retrospective analysis of the expression of STX6 in the paraffin samples of 147 EOC patients,the results showed that the expression of STX6 was significantly correlated with tumor differentiation,peritoneal cancer index(PCI),tumor stage(P<0.05),but not with the age of patients(P>0.05).And patients with high STX6 expression had a significant decrease in PFS expression(P<0.05),suggesting that STX6 may promote ovarian cancer progression and affect patient outcomes.(3)The detection of fresh tissue samples by q RT-PCR and immunoblotting showed that compared with normal ovarian tissue,STX6 in ovarian cancer tissue and peritoneal metastases showed a significant overexpression trend(P<0.05),and the expression of STX6 in peritoneal metastases was higher than that in ovarian cancer tissue(P<0.05).(4)After overexpression of STX6,the activity,proliferation ability,and migration ability of nest cancer cells were increased compared to the control group,while after silencing of STX6 expression,the activity,proliferation ability,and migration ability of ovarian cancer cells significantly decreased(P<0.05).Conclusion:(1)In ovarian cancer samples,STX6 is highly expressed,and the high expression of STX6 is significantly correlated with tumor differentiation,PCI,and tumor stage.(2)The expression level of STX6 is closely related to the prognosis of patients,and the prognosis of ovarian cancer patients with high expression of STX6 is even worse.(3)The overexpression of STX6 can enhance the activity,proliferation,and migration ability of ovarian cancer cells,thereby promoting the progression of epithelial ovarian cancer. |
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