Study On Stearic Acid Modified Nano CuMOFs And Their Loading And Sustained Release Of NO

Nitric oxide(NO)is a gaseous signaling molecule of the cardiovascular system with a variety of physiological functions including anticoagulant,antibacterial,endothelialpromoting and anti-inflammatory.NO is highly oxidable and can cause harm to the body in high concentrations,so it is difficult to treat by delivering it directly.Metal-organic Frameworks(MOFs)are a kind of organic-inorganic hybrid porous materials,which have great advantages in gas storage and many other fields such as biomedicine.However,the MOFs skeleton is unstable and easily damaged in a humid environment.In order to further improve the stability of MOFs skeleton.In this paper,super-hydrophobic copper based MOFs were synthesized and their capacity to load and release NO,as well as their anticoagulant and antibacterial functions were investigated.Firstly,the synthesis method of CuMOFs particles was explored,and CuMOFs nanoparticles were successfully synthesized using hydrothermal method.Then CuMOFs nanoparticles were synthesized and modified by stearic acid(SA)to increase their hydrophobicity and stability.Scanning electron microscopy(SEM)characterization results showed that the size of SA-modified CuMOFs were between 100-200 nm,and X-ray diffraction(XRD)characterization results showed that the structure of SA-modified CuMOFs were similar to HKUST-1.The contact angle measurement(WCA)results showed that the SA-modified CuMOFs were super-hydrophobic,and the Fourier transform infrared spectroscopy(FT-IR)characterization indicated that the SA modification was successful.Further,SA-modified CuMOFs adsorbed NO with the reaction of the amino group and NO to form a NONOates.The results showed that the release time of NO was up to 168 h,and the total release time of NO was 1.52 mmol/g.In vitro biological evaluation of stearic acid modified CuMOFs nanoparticles was carried out.The results showed that the SA-modified CuMOFs had good anticoagulant and antibacterial functions after loading NO.The anticoagulant properties were mainly manifested by their ability to slowly release NO under physiological environment,thus inhibiting platelet activation and adhesion,and inhibiting the adhesion of red blood cells.The good cytocompatibility was mainly manifested by the adhesion and proliferation of endothelial cells and macrophages were significantly promoted and inhibited,respectively.In summary,SA-modified CuMOFs nanoparticles were successfully synthesized,which have good stability and super-hydrophobicity and could adsorb NO with the reaction of the amino group and NO to form a NONOates.This thesis provides a new idea for the design of anticoagulant and antibacterial functions for blood contact materials.