| Background:Purslane is a medicinal plant known to have antioxidant,anti-inflammatory and anticancer activities and may also play an important role in colon cancerPurpose:To investigate the inhibitory effect of portulaca oleracea extract on colon cancer cells and its mechanism.Methods:Mouse experiment:(1)Colon cancer model construction: In this study,azomethane oxide and dextran sodium sulfate were used to induce the mouse colon cancer model.7-week-old female C57BL/6J mice were randomly divided into control group,modeling induction group,25mg/kg,50mg/kg and100mg/kg purslane extract groups.After 13 weeks of intervention,the general situation,feed intake,stool status,size and number of colorectal tumors of the mice were recorded.(2)The expression of C-My C and Cyclin D1 in colon cancer model tissues of mice in each group was detected by immunohistochemistry.(3)16S rRNA gene sequencing was used to analyze the changes of intestinal microbial diversity in each group.(4)Metabolite changes of large intestine in each group were analyzed by metabonomics sequencing.Cell experiments:(1)Human colon cancer cell HT-29 was randomly divided into three groups: Control group(complete culture medium),POE group(100 ug /ml POE added to cell culture medium),POE+ SKL2001 group(100 ug /ml POE and 30 u M SKL2001 added to cell culture medium),cell number and apoptosis were observed 24 hours after cell culture.(2)Western blot was used to detect the expression of C-My C and Cyclin D1 in each group.Results:After AOM/ DSS treatment,the body weight of mice decreased,the colon tissue was severely damaged,the colon microbiota was disturbed,and the harmful bacteria increased.After POE treatment,the mice regained weight,and the harmful bacteria decreased while the beneficial bacteria increased in a dose-dependent manner.In addition,we also found that POE can inhibit the proliferation of colon cancer cells by down-regulatting the expression of C-my C and Cyclin D1 in colon cancer cells,Conclusion:POE inhibits the progression of colon cancer by regulating the expression of C-my C and cyclin D1. |
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