| objective:To investigate the effect of intravoxel incoherent motion(IVIM),T1-mapping and T2-mapping functional magnetic resonance imaging techniques on evaluating renal microstructure and perfusion changes in intrauterine growth restriction(IUGR)SD rats.Methods:The neonatal IUGR SD rats were established by the low-protein diet during pregnancy as the experimental group,and the rats of normal protein diet during pregnancy were selected as the control group.At 8w and 12w after birth,each group was divided into normal 8w group,IUGR8w group,normal 8w group and IUGR 12w group.At the corresponding time points,the renal intra-voxel incoherent motion(IVIM),T1-mapping and T2-mapping magnetic resonance imaging scans were performed,and the images were analyzed by the synoptic processing station.IVIM images obtained ADC,D_t,D~*and f values of rat kidney cortex and medulla;T1-mapping images obtained T1 values;T2-mapping images obtained T2 values.After the completion of the magnetic resonance imaging scan,venous blood was collected from each rat to complete the detection of serum creatinine and blood urea nitrogen,and 2 rats were randomly selected from each group for pathological examination.SPSS 24.0 software was used to process experimental data,measurement data were expressed as mean±standard deviation((?)±s);t test was used for comparison between groups and Mann-Whitney U test was used for non-normally distributed data.One-way ANOVA was used to compare laboratory indicators.Comparisons of rates between groups were performed using theχ~2 test.P<0.05 considered the difference to be statistically significant.Result:1.The IUGR SD rats modeling successfully.2.There was no significant difference in rat’s renal ADC values between the cortex and medulla of each group and between each group(P>0.05).There was no significant difference in rat’s renal f values between the cortex and medulla of each group and between each group(P>0.05).The rat’s renal medulla D_t value of IUGR 12w group was higher than that of IUGR8w group,and the difference was statistically significant(P<0.05).The rat’s renal medulla D~*value of normal 12w group was higher than that of IUGR 12w group,the rat’s renal medulla D~*value of IUGR 8w group was higher than that of IUGR 12w group,and the difference were statistically significant(P<0.05).3.The rat’s renal medulla T1 value of the IUGR8w group was higher than that of the cortex,the rat’s renal medulla T1 value of the normal 12w group was lower than that of the IUGR 12w group,the rat’s renal cortex T1 value of the normal 8w group was higher than that of the normal 12w group,and the difference were statistically significant(P<0.05).4.The rat’s T2 value of medulla in each group was higher than that of cortex,and the difference was statistically significant(P<0.05);there was no significant difference in rat’s T2 value between groups(P>0.05).5.There was no significant difference in rat’s serum creatinine and blood urea nitrogen among the normal 8w group,IUGR 8w group,normal 12w group and IUGR 12w group(P>0.05).6.the pathological examination of the kidneys of rats of normal 8w group and normal 12w group shows no abnormality;the rats of IUGR 8w group showed glomerular hypertrophy without obvious fibrotic changes;the kidneys of rats of IUGR 12w group showed glomerular atrophy and cystic stenosis,interstitial infiltration of inflammatory cells and fibrosis.Conclusion:1.The serum creatinine and blood urea nitrogen of IUGR rats did not change significantly,but renal pathological examination showed glomerular hypertrophy,atrophy,sclerosis,renal interstitial inflammatory cell infiltration and even fibrosis at 12w.2.IVIM MRI is a method to evaluate and dynamically observe the microstructure and perfusion injury sensitivity of IUGR rat kidneys.3.T1-mapping MRI can be used to evaluate the kidney damage of IUGR rats;T1-mapping combined with T2-mapping MRI can futher distinguish renal fibrosis in IUGR rats.Figures 14;Tables 9;References 54... |
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