Components Analysis Of Chunru Ningjiao Based On UPLC-Q-TOF-MS And Mechanism Of Chronic Cervicitis By Network Pharmacology

Chunru Ningjiao is composed of five herbs,Chun Pi,Ku Shen,Mu Dan Pi,Ru Xiang and Bing Pian which have a significant curative effect in clinical treatment of chronic cervicitis,but its pharmacodynamic material basis and mechanism of action have not been deeply studied.In this study,UPLC-QTOF MS and GC-MS technologies were used to analyzes the chemical components contained in it,and preliminarily clarifies its pharmacodynamic material basis and mechanism of action,and provides a reference for rational clinical application.The main research content includes the following two parts:1.Identification and characterization of the chemical compounds of Chunru Ningjiao(1)The UPLC-QTOF MS analytical method of the Chunru Ningjiao was established.The composition information obtained in positive and negative ion mode,and identify the chemical composition by molecular weight,fragment ion peak and retention time,combined with literature and database information.A total of 102 compounds identified.(2)Establish the GC-MS analysis method of Chunru Ningjiao to identify the volatile chemical constituents in it.4 compounds were identified total.Finally,106 compounds were identified.Among them,57 compounds from Chun Pi(2 of which were shared with Ku Shen,2 were shared with Mu Dan Pi,and 2 were shared with Ru Xiang);44 compounds from Ku Shen(2 of which were shared with Chun Pi);4 compounds from Mu Dan Pi(2 of which were shared with Chun Pi);There are 5 compounds from Ru Xiang(2 of which were shared with Chun Pi);2 compounds from Bing Pian.2.Analysis of pharmacodynamic substances and mechanism of action of Chunru NingjiaoBased on the three component target databases of Swiss Target Prediction,SEA and Pharmmapper,and the three disease target databases of OMIM,Gene Cards,and Drug Bank,and combined with Venn diagram,the components contained in Chunru Ningjiao compound and the targets of chronic cervicitis were integrated.The intersection targets were annotated and visualized by Cytoscape software to build the “component-target-disease-metabolic pathway” correlation network.The network was analyzed using parameters such as degree values to obtain the most core components and targets of Chunru Ningjiao in the treatment of chronic cervicitis.Then,MOE molecular docking software was used to conduct molecular docking verification on the top 5 compounds and targets respectively.The results showed that ferulic acid,Shinjulactone A,Ailanthone,Shinjudilactone and Ficusesquilignan B may be the material basis for the anti-chronic cervicitis of Chunru Ningjiao.The treatment of chronic cervicitis may be achieved by regulating the level of sex hormones in vivo through estrogen,prolactin and other sex hormone signaling pathways;regulating PI3K-Akt signaling pathway,TNF signaling pathway,VEGF signaling pathway,etc.to regulate cell proliferation or apoptosis;regulating MAPK signaling pathway,B cell receptor Inflammation-related pathways such as signaling pathways to regulate cervical inflammation.Finally,the results of network pharmacology were verified by molecular docking,and the results showed that the core components had better binding activity to the core targets.The results showed that the top 5 core components ferulic acid,Shinjulactone A,Ailanthone,Shinjudilactone and Ficusesquilignan B had good binding to the top 5 core targets(MAPK1,ESR1,CDK2,MAPK8,MAPK14).This study reflects the principle of multi-component-multi-target-multi-pathway synergistic effect of the traditional Chinese medicine preparation Chunru Ningjiao.Figure 26,Table 9,References 88...