Regulation Of Active Components Of Traditional Chinese Medicine On Gut Strains

Background:Traditional Chinese Medicine(TCM)is a cultural treasure of the Chinese nation and has made significant contributions to the prevention and treatment of human diseases.However,the complexity of TCM components and the relative lack of modern scientific verification often hinder the research progress of TCM.Based on the principle of"multi-component versus multi-target" TCM therapy,more and more studies have found that gut microbiota,which are closely related to human health,are closely related to the pharmacodynamic relationship and pharmacodynamic substance basis of TCM.However,the current understanding of the interactions between various TCM and their active components and gut microbiota is still limited.Therefore,it is of great significance to accelerate the modernization of TCM to explore the interaction mechanism between gut microbiota and active ingredients of TCM,to find the target of gut microbiota by which Chinese medicine monomers play a role,and to clarify the molecular mechanism by which Chinese medicine monomers mediate the effect of gut microbiota.Objective:To systematically explore the effects of various Chinese medicine monomers on the growth of various gut core strains,and summarize the effects of Chinese medicine monomers on gut strains.Methods:Firstly,a screening database of 407 Chinese medicine monomers in 18 categories and a database of 47 core gut strains were constructed.Relying on high-throughput screening platform,the effect of each Chinese medicine monomers on the growth of each gut strain was detected by OD600 value of enzyme plate with 384 well microplate at the concentration of 20μM.The effects of drugs on relative abundance of key strains were detected by fluorescence quantitative PCR.In addition,the main factors affecting the antibacterial effect of Chinese medicine monomers were studied by random forest analysis.Results:In this study,it was found that among 407 monomers screened,56 monomers(13.8%)affected the growth of at least one human core gut strain(q value≤0.01).Quinone compounds(12/15)had the most extensive effect on the strain and had significant antibacterial effect.In addition,13 Chinese medicine monomers(Inhibitory strains≥5)that may have extensive anti-symbiotic bacteria activity were screened,including:Chelerythrine(chloride),Aristolochic acid A,Ginsenoside CK,20(S)Ginsenoside Rh2,Glycycoumarin,Glyasperina,Isobavachalcone,Rhein,Chrysophanol,Danthron,Aloe emodin,Myristic acid,1-Monomyristin.And the inhibition value of 12 Chinese medicine monomers on some strains was over 90%,suggesting that these Chinese medicine monomers had specific inhibitory effect on some strains.Through random forest analysis of chemical properties and molecular characteristics of traditional Chinese medicine monomers against symbiotic bacteria,it is found that chemical characteristics(Xlogp,TPSA,Complexity,DASC,HBDC)and molecular characteristics(AATSlp,AATSC7m,AATSC7Z,MATS7are,MATS7m)may be the main characteristics determining the anti symbiotic activity of traditional Chinese Medicine monomers.Conclusion:In this study,it was found that Chinese medicine monomers with various structural types had significant inhibitory effects on core gut strains,Quinones have general bacteriostasis,followed by Triterpenoids,Alkaloids,Terpenoids,Phenylpropanoids,Flavonoids and Fatty acids,while Saccharides,Glycosides,Aldehydes and Steroids have little effect,which provided reference for TCM mediated gut microbiota to exert efficacy.