Research Progress Of Melatonin Promoting Oxidative Damage Of Gastric Cancer By Regulating MiRNA-29b-3p

Objective:Melatonin(MT)has anti-tumor effect,but its specific anti-tumor potential mechanism is still unclear.In this paper,we verified the important role of MT in gastric cancer cells through a variety of experiments,and further explored its potential molecular mechanism and explored its possibility as an anticancer drug.Methods:1.The effects of different concentrations of MT on SGC-7901 gastric cancer cell model were constructed to observe the effect of MT on the growth of gastric cancer cells.The optimal concentration and time of MT on gastric cancer cells were screened by CCK8 method for subsequent treatment of gastric cancer cells.2.The differential m RNAs after MT treatment of gastric cancer cells were screened by high-throughput sequencing,and the biological functions related to oxidation and the corresponding mi RNA were obtained by bioinformatics analysis,and the targeting relationship and binding region of mi RNA and m RNA were predicted.3.The relationship between the key mi RNA-29b-3p and the target gene PDGFRB was determined by q RT-PCR,and the expression levels of mi RNA-29b-3p and the target gene PDGFRB in MT-treated gastric cancer cells were verified by double luciferase assay,q RT-PCR and Western blot.Results:1.Melatonin can inhibit the growth and proliferation of human gastric cancer cell SGC-7901,and is proportional to the concentration and time of melatonin(p<0.05).2.High-throughput sequencing and bioinformatics analysis showed that mi RNA-29b-3p and the target gene PDGFRB were involved in the oxidative damage of gastric cancer cells in melatonin-treated gastric cancer cell lines.3.The dual luciferase assay verified that mi R-29b-3p could bind to the 3UTR of PDGFRB and down-regulate the target gene PDGFRB.The results of q RT-PCR and Western blot showed that melatonin inhibited the proliferation of gastric cancer cells by enhancing the expression of mi R-29b-3p and reducing the level of PDGFRB.Conclusion:Melatonin down-regulates the level of target gene PDGFRB by enhancing the expression of mi RNA-29b-3p in gastric cancer cells,thereby promoting oxidative damage of gastric cancer cells and ultimately inhibiting the proliferation of gastric cancer cells.