Changes Of Cytokine Profile In Aqueous Humor Of Neovascular Glaucoma After Vitrectomy In Eyes With PDR

Objective:To analyze the concentration of cytokines in aqueous humor of neovascular glaucoma after vitrectomy in eyes with proliferative diabetic retinopathy.To provide new ideas to explore the pathogenesis of neovascular glaucoma after vitrectomy,and to guide clinical practice.Methods:A prospective analysis was performed on 19 patients with NVG secondary to PDR who were treated in our hospital from October 2021 to November 2022.According to whether they accepted parsplannavitrecomy,they were divided into the NVG after vitrectomy group(8 cases)and the control group(11 cases).The baseline data and clinical surgical indicators were collected and recorded for each patient in both groups:preoperative baseline data included gender,age,duration of type II diabetes,history of hypertension,and glycated hemoglobin level;ocular baseline data included baseline IOP,iris neovascularization,whether pan-retinal photocoagulation,anti-VEGF treatment was completed before surgery,whether the eye with IOL,whether PPV with cataract surgery,internal limiting membrane peeling and silicone oil tamponade,whether other postoperative complications were combined.In both groups,100 μl of aqueous humor was drawn before intravitreal injection.Luminex method was used to determined the concentration of various cytokines in aqueous humor.Include: IP-10、IFN-α、IFN-γ、MCP-1、ICAM-1、MCP-3、MIG、、G-CSF、Eotaxin、MIP-1β、MIP-3β、CCL22、EGF、FGFbasic、VEGF、VEGFR2、PDGF-AA、HGF、TNF-α、IL-1β、IL-2、IL-4、IL-5、IL-6、IL-8、IL-10 和 IL-12.To analyze the relationship between baseline data,clinical surgical indicators and cytokines concentration.SPSS26.0 software was used for statistical analysis.Results:1.The concentration of cytokines in the aqueous humor of the the NVG after vitrectomy group and the control group were compared.The concentration of cytokines VEGFR2 and MCP-1 in the aqueous humor of the the NVG after vitrectomy group were statistically different from those in the aqueous humor of the control group(P<0.05).There was no stastical difference in other cytokines(P>0.05).2.In the NVG after vitrectomy group,the concentration of cytokines CCL22、IL-5、ICAM-1、HGF、MIP-3β and MIP-1β were positively correlated with VEGFR2(P<0.05).The concentration of cytokines PDGF-AA、IL-1β、IL-2、IL-4、IL-8、MIG、MCP-3、TNF-α、MIP-1β、IFN-γ and Eotaxin were positively correlated with VEGF(P< 0.05).MCP-1 was no positively correlated with the concentration of other cytokines(P>0.05).3.In the NVG after vitrectomy group,the concentration of cytokines TNF-α、MIG、MCP-3、MIP-1β、IL-1β、IL-2、IL-12 and IL-8 were positively correlated with preoperative baseline IOP(P < 0.05).The concentration of cytokines IL-6 was negatively correlated with e GFR levels(P<0.05).The concentration of cytokines ICAM-1 was positively correlated with PPV with cataract surgery(P<0.01).4.In the NVG after vitrectomy group,the other baseline data and clinical surgical indicators were no correlated with the concentration of cytokines(P>0.05).Conclusion:1.In the NVG after vitrectomy group,the concentration of cytokines VEGFR2 and MCP-1 were significantly higher.2.The occurrence of NVG after vitrectomy is mediated by multiple cytokines,which interact with each other through complex mechanisms.3.The higher baseline IOP was,the higher the concentration of TNF-α、MIG、MCP-3、MIP-1β、IL-1β、IL-12、IL-8 and IL-2 in the aqueous humor of the the NVG after vitrectomy group.4.The lower the e GFR level,the higher the the concentration of IL-6 in the aqueous humor of the the NVG after vitrectomy group.5.PPV combined with cataract surgery increase ICAM-1 concentrations in the aqueous humor.