Glutamine Regulates Inflammation Through 14-3-3 Protein

Background:Sepsis is a kind of life-threatening multiple organ dysfunction syndrome(multipleorgandysfunctionsyndrome,MODS)caused by infection response disorder,which has the characteristics of high incidence and high mortality.As one of the pathophysiological mechanisms of sepsis,inflammatory imbalance plays a very important role in sepsis.Glutamine is the most abundant and widely used amino acid in human body.Nowadays,glutamine has been widely studied,and it has been found that glutamine can protect the morphology and corresponding function of organs during sepsis,and reduce a series of important organs such as brain,heart,liver,intestine and kidney.Timely and effective glutamine supplementation can reduce inflammatory response and inhibit the release of inflammatory factors.14-3-3 protein family is a kind of highly conserved cellular protein family,which is widely expressed in all eukaryotes.Current studies have also found that 14-3-3 protein is closely related to inflammation,but whether the anti-inflammatory mechanism of glutamine is related to 14-3-3 protein remains to be studied.MethodsMacrophage inflammation model was established by stimulating RAW264.7macrophages with LPS,and the changes of 14-3-3 protein were observed by adding glutamine.Then lentivirus interferes with the expression of 14-3-3m RNA to reduce the expression of 14-3-3 protein.The expression level of 14-3-3 gene was detected by RTq PCR.The expression of ERK1/2,P-ERK1/2,JNK,P-JNK,p38,P-p38,NF-κ B and PMI NF-κ B was detected by WB。Results1.Glutamine can cause overexpression of 14-3-3 protein in RAW264.7 cells stimulated by LPS2.Glutamine inhibits inflammatory response by overexpression of 14-3-3 protein to inhibit MAPK signal pathway.ConclusionThis study demonstrated the mechanism of glutamine and 14-3-3 protein regulating inflammation in MAPK signal pathway in vitro.It was found that:(1)Glutamine can induce the overexpression of 14-3-3 protein in RAW264.7 cells stimulated by LPS.(2)the anti-inflammatory effect of glutamine is realized by 14-3-3protein inhibiting MAPK signal pathway.