A Clinical Research: Vascular Degree Of Artificial Dermis Detected By Laser Speckle Contrast Imaging Influence The Therapeutic Effect For Wound Healing

Objective:To observe blood perfusion on wounds by laser scatter contrast imaging（LSCI）technology,and exploring the effect of vascular degree on wound efficacy in burn wounds of artificial dermal.Methods:The clinical information of 27 burn trauma victims who qualified for screening were collected from the First Affiliated Hospital of Nanchang University from 01,2022to 04,2023,in parallel with a retrospective cohort study.According to the trauma bloodstream perfusion scores measured by LSCI instrument,9 patients were included in the low-visualization group[8 males and 1 female,age（47.56±20.58）years,artificial dermal coverage trauma area 60（45,90）cm²];10 patients were included in the middle-visualization group[5 males and 5 females,age（41.80±14.74）years,artificial dermal coverage trauma area 50(40,（92.5）cm²];8 patients were included in the high-visualization group[7 males and 1 female,age（44.25±7.59）years,artificial dermal coverage of 40（22.5,112.5）cm²].All patients of the three groups were compared in terms of the ROI_gola ratio within 24 hours before split skin grafting,the artificial dermis vascular period,the survival rate on 7th day after split skin grafting,the length of hospital stays,the hospitalization costs and the VSS scores for goal area within three months after the split skin grafting surgery.Quantitative data:Normality distribution tests were performed,with normally distributed data expressed as means±standard deviations?±S)and skewed or variance-deficient data expressed as median and interquartile spacing M（P25,P75）.Qualitative data:expressed as cases（n）,multiple group rates and composition ratios were compared.The SPSS 27.0 software was used to analyse the data.Results:1)The ROI_gola ratio within 24 hours before skin grafting:120.53（103.68,120.61）%in the low-visualization group,199.72（162.84,251.40）%in an middle-visualization group,and 352.10（346.98,363.98）%in the high-visualization group.The ROI_goalratio（high-visualization group）>the ROI_goal ratio（the middle-visualization group）>the ROI_goal ratio（low-visualization group）,the difference was statistically meaningful（P<0.05）.2)The artificial dermis vascular period:（16.0±6.7）days in the low-visualization group,（15.6±3.02）days in the middle-visualization group and（23.25±6.45）days in the high-visualization group;the artificial dermis vascularization period in both the middle-visualization group and low-visualization group were shorter than in the high-visualization group,and the difference was statistically significant（P<0.05）.3)The survival rate on 7th day after split skin grafting:（89.89±3.551）%in the low-visualization group,（94.80±3.393）%in the middle-visualization group and（94.00±4.342）%in the high-visualization group;the survival rate on 7th day after split skin grafting in both the middle-visualization group and high-visualization group were higher than in the low-visualization group,and the difference was statistically significant（P<0.05）.4)The length of hospital stays:（55.11±17.113）days in the low-visualization group,（36.20±7.239）days in the middle-visualization group and（42.38±8.434）days in the high-visualization group.The length of hospital stays in both the middle-visualization group and the high-visualization group were shorter than in the low-visualization group,and the difference was statistically significant（P<0.05）.5)The hospitalization costs:（14..08±9.08）,000 yuan in the low-visualization group,（113.9±7.75）,000 yuan in the observation group and（130.8±11.18）,000 yuan in the high-visualization group;the hospitalization costs were generally approaching among the three groups and not obviously different in statistics（F=0.203,P=0.817）.6)The VSS scores for goal area within three months after the split skin grafting surgery:5（4.0,5.5）in the low-visualization group,3（2.75,3.0）in the middle-visualization group and 3.5（3.0,4.75）in the high-visualization group;the VSS scores in the middle-visualization group was lower than that in the low-visualization group,and the difference was statistically meaningful（P<0.05）.Conclusion:The using of LSCI technique to observe the degree of artificial dermal vascularization may be one of the methods assessing the degree of artificial dermal vascularization,which is beneficial to reduce unnecessary waiting time and guiding the timing of autologous skin grafting after the completion of artificial dermal vascularization,and improving the healing quality of wounds,and envolving favourable application perspectives.