| Purpose To investigate the expression of gap connexin 32(Cx32)and phosphorylated protein kinase B(p-AKT)in endometrial carcinoma tissues and their association with the pathological features of endometrial carcinoma.And to explore their association with the pathological features of endometrial carcinoma,so as to provide new ideas for the diagnosis and treatment of endometrial carcinoma.Method Samples of 40 patients who underwent hysterectomy for endometrial carcinoma in our hospital from May 2017 to May 2021 were collected.None of them underwent preoperative chemoradiotherapy or immunotherapy.On this basis,samples of 20 patients who underwent hysterectomy due to uterine fibroids/uterine prolapse during the same period and were confirmed as endometrium of normal hyperplasia by postoperative pathology were selected.Clinical variables were recorded,including age,degree of differentiation,muscular invasion,lymph node metastasis,vascular invasion,and clinical stage.Histological grading according to Edmondson-Steiner(ES)criteria showed 33(82.5%)highly differentiated tumors and 7(17.5%)poorly differentiated tumors.According to the International Federation of Obstetrics and Gynecology(FIGO,2014),surgical pathological staging showed that there were 24 patients(60.0%)with stage Ⅰ-Ⅱ and 16 patients(40.0%)with stage Ⅲ-Ⅳ tumors.The expression of Cx32 and p-AKT was detected in 40 tissues of endometrium and 20 normal hyperplasia endometrial tissue by immunohistochemical method,and the relationship between the expression levels and clinicopathologic parameters was analyzed.Result 1.Cx32 showed obvious membrane localization in endometrial tissue during normal hyperplasia.In endometrial carcinoma tissues,Cx32 showed cytoplasmic localization,and its expression was relatively low.p-AKT positive signal is located in cytoplasm and nucleus,and the expression of P-Akt is increased in carcinoma tissues.2.The positive expression rate of Cx32 was 62.50%(25/40)in endometrial carcinoma tissue and 100.00%(20/20)in normal hyperplasia endometrial tissue,and there were significant differences between the two groups(P<0.05).The positive expression rate of p-AKT was 85.00%(34/40)in endometrial carcinoma tissue and 35.00%(7/20)in normal hyperplasia endometrial tissue,and there were significant differences between the two groups(P<0.05).3.Cx32 was negatively correlated with the expression of p-AKT in endometrial carcinoma(rs=-0.325,P<0.05).4.There was a significant correlation between Cx32 expression in endometrial carcinoma tissues and the degree of differentiation,the depth of invasion into myometrium,the presence or absence of lymph node metastasis,and the clinical stage of the disease(P<0.05),but was not significantly correlated with age and presence or absence of vascular invasion(P>0.05).There was a significant correlation between the p-AKT expression in endometrial carcinoma and the degree of infiltration of myometrium(P<0.05),but there was no significant correlation between age,degree of differentiation,lymph node metastasis,presentation of vascular invasion,and clinical stage(P>0.05).Conclusion 1.Compared with normal hyperplasia endometrial,the expression of Cx32 decreased,and the expression of p-AKT increased,and there was a negative correlation between Cx32 expression and development of endometrial carcinoma,and the two may play opposite roles in the biological behavior of endometrial carcinoma.Cx32 may be a tumor suppressor factor and a potential molecular marker for endometrial carcinoma, which may provide a new idea for the diagnosis and targeted therapy of endometrial carcinoma.2.The correlation of Cx32 expression in endometrial carcinoma with the degree of differentiation,the depth of invasion into myometrium,the presence of lymph node metastasis,and the clinical stage is conducive to further research on new molecular targets of endometrial carcinoma and become a potential marker for targeted therapy. |
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