Association Of Dimorphism In P2Y1 And P2Y12 Gene With Acute Myocardial Infarction And Platelet Aggregation Function

ObjectiveADP is an important mediator of platelet aggregation,which regulates the activation and aggregation of platelets through P2 Y receptors.Multiplex genotypes of P2 Y recepter could function one stimulate signal into different types,and make platelets in response to ADP activation in receptor genotypes dependently.In this study,we investigated the genotypes of ADP receptor and the relationship between ADP receptor genotypes,including P2Y1 and P2Y12,and AMI were explored,in the Han population in Quanzhou,Fujian Province.Important,data from our study will provide laboratory evidence for clinical acute myocardial infarction protection.MethodsThe study subjects were selected from the Department of Cardiovascular Medicine,Quanzhou First Hospital affiliated to Fujian Medical Universit and deliver into including control group(n=100)and AMI group(n=194).PL series multiparameter platelet function analyzer was used to detect the maximum platelet aggregation rate(MAR).Serum levels of GLU,BUN,CRE,TC,TG,HDL-C,LDL-C,Apo AI and Apo B were determined by AU5800 automatic biochemical analyzer.The DNA extracted from peripheral blood was amplified and sequenced using P2Y1 and P2Y12 SNP(including P2Y1rs701265(1622A>G),P2Y12rs6785930(34C>T),P2Y12rs6809699(52G>T),P2Y12rs10935838(i-139C>T)and rs2046934(i-744T>C)sites)special primer pairs.Statistical analysis was performed using SPSS 26.0 software.Results1.The BMI,SBP,DBP,CRE,GLU and TG levels were significantly increased,while the HDL-C and Apo AI levels were markedly lower,in AMI group.2.The polymorphic distribution of P2Y1(rs701265),P2Y12(rs6785930,rs6809699,rs10935838 and rs2046934)were accorded with H-W genetic balance(P>0.05),and the samples were representative of the population.3.There were no significant difference in the distribution frequency of SNP sites(including P2Y1rs701265,P2Y12rs6785930 and P2Y12rs6809699)(P>0.05).4.The T allele frequency of rs10935838 in the AMI group was higher than it in the control group(15.5% vs.8.76%,P<0.05),and as well it showed in the C allele rs20469342(18.82%vs11.22%,P<0.05).5.Logistic regression analysis indicate that the BMI,GLU and history of hypertension,may induce AMI,contrary HDL-C will reduce AMI risk.Furthermore both rs1093583 allelic CT/TT and rs2046934 TC/CC of P2Y12 were risk factor of AMI.6.The maximum platelet aggregation rate(MAR)in both rs10935838 and rs2046934 genotypes of P2Y12 were statistically different,in control group(P<0.05).Conclusions1.The distribution frequency of P2Y12rs6785930,P2Y12rs6809699 and P2Y1rs701265 in AMI was no sense while compaire with control group,indicating that such genotypes may not related to the occurrence of AMI.2.The distribution frequency of both rs1093583 and rs2046934 P2Y12 SNP site were statistical different between AMI and control group.Both allelic CT/TT and TC/CC genotypes contributed the increasing risk of AMI.3.P2Y12rs1093583,P2Y12rs2046934 polymorphisms were related to platelet hyperresponsiveness.