Effects Of TIM-3 On Polarization Of Macrophages And Proliferation Of A549 Cells

Background:Lung cancer is the main malignant tumor that endangers human health.Its morbidity ranks second in the world,and its mortality ranks first.Among these patients about 85% belong to non-small-cell lung cancer(NSCLC).Currently,surgical operation is still the main treatment for resectable lung cancer,which only accounts for about30% to 40% of NSCLC.In recent years,with the development of tumor immunotherapy,the treatment of lung cancer has made a new progress.Macrophages are an important immune component in tumor microenvironment(TME),and play an important role in the derivation and development of tumor.T cell immunoglobulin and mucin-domain-containing molecules 3(Tim-3)is a kind of immune checkpoint,which is also expressed in macrophages.Verification of the effect of Tim-3 on macrophage polarization and the resulting effect on the proliferation of non-small cell lung cancer A549 cells will open up a new way for the immunotherapy of lung cancer.Objective:To verify the relationship between Tim-3 and macrophage polarization,and observe its effect on proliferation of A549 cells.Methods:1.To explore the relationship between Tim-3 and the polarization of macrophages,we decreased the expression of Tim-3 in macrophages with siTim-3,and then detected the expression levels of iNOS,CD80,CD163 and Arg-1 in macrophages by qRT-PCR and Western Blot.2.To explore the effect on the proliferation of A549 cells,we collected macrophage supernatant after siTim-3’interference and then mixed it with a certain proportion of complete medium to stimulate the A549 cells.The proliferation of A549 cells within 72 hours was detected by CCK8 cell proliferation test.Results:1.Aftering decreasing the expression of Tim-3 in macrophages,the expression of M1 type molecules CD80 and iNOS were significantly increased,while the expression of M2 type molecules CD163 and Arg-1 were highly decreased.2.The supernatant of macrophages interfered by siTim-3 had no significant effect on the proliferation of A549 cells.Conclusions:Tim-3 plays a role in the polarization of macrophage and promotes the polarization of macrophages to M2.The expression level of Tim-3 in macrophages has no significant effect on the proliferation of A549.