Effects Of Overexpression Of CENPI Gene On Biological Behavior Of Gastric Cancer SGC7901 Cells

Objective: To investigate the effects of CENPI gene overexpression on cell proliferation,migration,invasion and apoptosis in SGC7901 gastric cancer cells.Methods: In this study,human gastric cancer SGC7901 cells were firstly cultured in vitro and lentivirus gene expression regulation technique was used to construct lentivirus negative control(NC)and CENPI gene overexpression(OE).After 72 hours,the transfection efficiency was observed under fluorescence microscopy.The expression level of CENPI m RNA in the group was detected by real-time fluorescence quantitative polymerase chain reaction(QRT-PCR).Finally,MTS assay and colony formation assay were used to detect cell proliferation.wound healing and Transwell chamber with or without Matrigel was utilized to detect the migration and invasion of cells,and Western Blot was applied to measure the expression of apoptosis-associated protein PARP.Results: 1.After transfection of gastric cancer SGC7901 cells with lentivirus for 72 h,the abundance of fluorescence expression was more than90%.2.The results of QRT-PCR showed that the CENPI m RNA expression level in the overexpressed OE group was significantly higher than that in the NC group and non-transfection group(P <0.001),Accordingly,the OE group and the NC group were selected for subsequent experiments.3.The absorbance value(OD value)of two group at 24 h,48h,72 h,96h and 120 h was analyzed by MTS cell proliferation test and accordingly calculated cell viability.Compared with the NC,there was significant in OE group from 72 h to 120h(P <0.001).Furthermore,Statistical analysis of the number of clones formed by clone formation experiment showed that the number of clones formed by OE group was much more than that of NC group(P <0.001).4.The results of the wound healing assay confirmed that the cell migration rate was increased distinctly in the OE group compared with NC group at 24 h and 48h(P<0.01).5.The results of transwell chamber(with or without Matrigel)experiments both validated that the number of migration and invasion cells in OE group was much more than that in NC group(P< 0.001).6.Western Blot results showed that relative expression level of associated-apoptosis protein PARP in overexpressed OE group was significantly decrease compared with the NC group(P < 0.001).Conclusions: Overexpression of CENPI gene promotes proliferation,migration and invasion of gastric cancer SGC7901 cells,and may inhibit apoptosis.