| Objectives The purpose of this study was to investigate the improvement effect of Sanghuang Tongxie Formula(SHTXF)on heart mass index,blood glucose,oxidative stress and myocardial injury and the expression of PI3 K,Akt,p-AKT,m TOR,p-m TOR,Beclin-1 and LC3 protein and heart tissue pathological in type 2 diabetes model rats and and explore the potential molecular mechanism.Methods The 60 SD male rats of 5 ～ 6-week-old were selected for adaptive feeding for one week.Ten of rats were selected randomly as regular group and they were fed with regular feeding,while the others were fed with high-glucose-high-fat diet as experimental group.After 4 weeks,the experimental group of rats were fasted and cannot help water for12 hours,after 12 hours injection STZ(30mg/kg),at the same time the regular group was given the equal amounts of regular saline.The rats were closely observed for 72 hours after the injection.After 72 hours,the modeled rats were subjected to oral glucose tolerance test,with FBG > 11.1 mmol/L or PG2 h > 16.7 mmol/L as the modeling standard.The modeled rats were randomly divided into 5 groups: model group,metformin group and Sanghuang Tongxie Formula low-medium-high-dose group.The low-dose,medium-dose and high-dose groups were given 14g/kg,7g/kg and 3.5g/kg once a day intragastric administration of Sanghuang Tongxie Formula(the adult dose of Sanghuang Tongxie Formula was 70g/d,and the rat dose was converted according to body surface area).The normal group and the model group were given equal dose of distilled water,and the positive group was given metformin 0.2g/kg once a day for 12 weeks.After giving the rats the drug,the fasting blood glucose and the glucose tolerance were tested every other week.Twelve consecutive weeks after administration,the rats of heart weight and body weight were measured and then we should compute the heart mass index.Glycosylated hemoglobin,oxidative stress indexes and myocardial injury were detected.HE and VG staining were used to observe the myocardial structure and fibrosis.Western blot was used to detect the expressions of PI3 K,Akt,p-AKT,m TOR,p-m TOR,Beclin-1 and LC3 in myocardial tissue.Results 1 In the model group,fasting blood glucose,two hours postprandial blood glucose,glycated hemoglobin and heart mass index increased(P < 0.01).Compared with the model group,SHTXF could reduce fasting blood glucose,two hours postprandial blood glucose and glycosylated hemoglobin and heart mass index(P < 0.05),(P < 0.01).2 In the model group rats malondialdehyde(MDA)levels rose and superoxide dismutase(SOD)levels declined(P < 0.01),while after the treat-ment of SHTXF,the rats MDA levels declined and the SOD levels rose(P < 0.01).3 The level of creatine kinase-isozyme and lactate dehydrogenase in type 2 diabetes model rats were increased(P < 0.01);After treatment the level of them in the SHTXF group decreased(P < 0.05).4 HE staining and VG staining of heart tissue showed that compared with the model group,SHTXF could improve myocardial tissue structure and fibrosis degree of the heart tissue.5 Western Blot showed that in the model group,the expression of PI3K、p-Akt、p-m TOR increased(P <0.01),Beclin-1 and LC3 decreased(P < 0.01);Compared with the model group,SHTXF reduced the expression of PI3K、p-Akt、p-m TOR(P < 0.05),(P < 0.01)and increased Beclin-1 and LC3(P < 0.05).Conclusions 1 SHTXF can reduce the blood glucose level and heart mass index in T2 DM rats.2 SHTXF can down-regulate myocardial injury index in T2 DM rats and improve the myocardial tissue structure and fibrosis degree of the heart tissue of T2 DM rats.3 SHTXF can improve the myocardial injury of T2 DM rats,and its mechanism may be related to improve oxidative stress state of T2 DM rats and adjust the autophagy.Figure 5;Table 8;Reference 131... |
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