Bioinformatics Analysis Of Potentially Key Genes And Pathways In Castration-Resistant Prostate Cancer

Objectives Bioinformatics methods were used to explore the key genes and pathways of castration-resistant prostate cancer（CRPC）,and provide new ideas for its pathogenesis and treatment.Methods Data sets of human primary PCa and CRPC were downloaded from the Gene Expression Omnibus（GEO）database for bioinformatics analysis.Differentially expressed genes（DEGs）were identified using R language.Gene Ontology（GO）enrichment analysis and Kyoto Encyclopedia of Genes and Genomes（KEGG）pathway analysis were performed on DEGs using the DAVID software.A protein-protein interaction（PPI）network was constructed using STRING online database to further screen key genes.Survival analysis and Receiver Operating characteristic curve（ROC）analysis of key genes were conducted.Finally,DU145 cells were treated with Zn Cl₂,which is associated with important pathways,to investigate the effect of Zinc ion(Zn²⁺)on CRPC.And the experiment was divided into two groups:control group and experimental group,Cell proliferation was detected by CCK8 assay,cell migration was detected by scratch assay,and cell migration and invasion were detected by Transwell assay.Results 1 The authors found that a total of 279 DEGs were screened out through data set analysis.Further GO and KEGG analysis showed that signaling pathways such as cell division,mitosis and cell cycle play an important role in the development of CRPC.2 PPI network analysis screened out 15 key genes,survival analysis of key genes showed that the overall survival rate and disease-free survival rate of CRPC patients with high expression of CDC20,MAD2L1 and NUSAP1 were lower than those with low expression of CDC20,MAD2L1 and NUSAP1（P<0.05）.The areas under ROC curve for CDC20,MAD2L1 and NUSAP1 to predict the occurrence of CPRC were 0.933,0.762 and 0.950,respectively,suggesting that CDC20,MAD2L1 and NUSAP1 have high diagnostic value for CRPC.3After 24 hours of cell culture,the proliferation,migration and invasion rates of DU145cells treated with Zn Cl₂decreased（P<0.05）.Conclusions 1 CDC20,MAD2L1 and NUSAP1 may be key candidate genes involved in the development of CRPC.2 Zn²⁺may be a potential drug to inhibit the development of CRPC.3 Mitosis and cell cycle are important pathways affecting the development of CRPC.Figure 12;Table 4;Reference 261...