Molecular Mechanism Of Portal Vein Tumor Thrombus Formation In Hepatocellular Carcinoma

Objectives To explore the molecular mechanism of PVTT formation in HCC.Methods Part I: We collected 3 cases of PVTT tissues from HCC patients with their adjacent normal tissues for control groups.Total RNA was extracted and RNA-Seq technology was adopted to detect differentially expressed m RNA.These genes were put into Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)to show the possible mechanisms of formation of PVTT.Part II: To investigate the expression of m RNA and vascular endothelial growth factor(VEGF)protein in hepatocellular carcinoma and other tissues by immunohistochemistry and bioinformation analysis.To investigate the relationship between VEGF and tumor development and clinical prognosis,and to analyze the genes co-expressed with VEGF by GO and KEGG,so as to explore the signaling pathway involved in VEGF in hepatocellular carcinoma cells and its potential molecular biological mechanism.Results Part I: A total of 7233 differentially expressed coding genes were obtained by analysis of RNA-Seq results,of which 3700 were up-regulated and 3533 were downregulated in portal vein tumor thrombus of liver cancer patients,enrichment analysis showed that these genes were involved in mang cell life processes and many cancer-related pathways.Part II: VEGF was positively expressed in both HCC and normal liver tissues.In HCC patients,higher-expressed VEGF could indicate poor prognosis with statistically significant.VEGF participates in various progress in vivo,such as cell survival,actin recognization,cytoskeletal rearangment,cell migration,gene expression,cell proliferation and vascular cell permeability,in order to effect the ability of angiogenesis.Conclusions 1.Abnormal expression of a large number of m RNA in portal vein thrombus tissue of HCC patients.2.VEGF expression can be seen in both liver cancer and normal liver tissues.The overall survival rate and disease-free survival rate of patients with high VEGF expression are worse.Figure31;Table8;Reference 55...