The Correlation Between TNF-α,TGF-β₁,IL-6 And Respiratory Function In Patients With Pulmonary Fibrosis

BackgroundA variety of lung diseases can cause inflammatory damage to lung tissue,a large number of fibroblasts proliferation,extracellular matrix deposition,normal lung tissue was destroyed,abnormal repair of lung tissue structure abnormalities,resulting in Pulmonary fibrosis（PF）.Pulmonary fibrosis is the final outcome of many chronic lung diseases,mostly after middle age,male incidence.At present,due to the various causes of these diseases,there is no specific treatment drugs and methods,which is a difficult point in diagnosis and treatment of respiratory diseases.To conquer the diagnostic difficulties in many domestic and foreign scholars studied the pathogenesis of pulmonary fibrosis,in the majority with animal experiments,mostly using bleomycin induced pulmonary fibrosis in mice or rats model,the experiment confirmed pulmonary fibrosis development and Interleukin 1（IL-Interleukin-1,1）,Interleukin-6（Interleukin-6,IL-6）,Interleukin 8（Interleukin-8,IL-8）,Interleukin-13（Interleukin-13,Il-13）,interleukin-17（il-17）,Vascular endothelial growth factor（VEGF）,interferon-hormone（inf-hormone）,connective tissue growth factor（CTGF）and other cytokines are involved.ObjectiveThis study measured the pulmonary fibrosis patients serum TNF alpha,TGF-beta 1,IL-6 cytokine levels,detection of the arterial blood oxygen partial pressure and arterial blood co2 partial pressure,and detection of forced expiratory volume in one second and lung capacity,to explore the serum TNF alpha,TFG-beta 1,IL-6 level and the correlation of pulmonary fibrosis,serum TNF alpha,TGF beta 1,IL 6 level and the correlation of the respiratory function in patients with pulmonary fibrosis index,hoping to find a more reasonable joint detection index,for the clinical diagnosis and treatment of pulmonary fibrosis provides new train of thought.MethodsIn clinical practice,79 patients with confirmed pulmonary fibrosis were selected as the pulmonary fibrosis group,and 50 healthy subjects without pulmonary disease in physical examination were selected as the control group（excluding various pulmonary diseases,tumors,infections,etc.,and no abnormalities were found in chest radiographs and pulmonary function tests）.Proceed as follows:1.Test arterial partial oxygen pressure and partial carbon dioxide pressure;2.The levels of TNF-α,TFG-β₁ and IL-6 in venous blood of the two groups were measured by elisa double antibody sandwich method;3.The lung function tester measures Forced expiratory volume in one second（FEV₁）and Forced vital capacity（FVC）;Statistical methods:data were recorded as（mean±standard deviation）,t test was used for comparison between two groups,F test was used for comparison of mean between multiple groups,and correlation analysis was used for statistical analysis of correlation degree.Significant level was set as alpha=0.05,and P<0.05 was considered statistically significant.Results1.The levels of TNF-α,TFG-β₁ and IL-6 in venous blood of patients with pulmonary fibrosis were higher than those of the control group（P<0.05）,with statistical significance.2.Pulmonary fibrosis group had lower arterial oxygen partial pressure and higher carbon dioxide partial pressure than the control group,and the degree of hypoxia was positively correlated with the levels of serum TNF-α,TFG-β₁ and IL-6（P<0.05）,with statistical significance.3.Forced expiratory volume and lung capacity in one second in patients with pulmonary fibrosis were smaller than those in the control group,and their levels were negatively correlated with serum TNF-α,TFG-β₁ and IL-6 levels（P<0.05）,with statistical significance.ConclusionIn patients with pulmonary fibrosis,Pa CO₂ was increased,and serum TNF-α,TFG-β₁and IL-6 levels were positively correlated with Pa O₂ decrease,and negatively correlated with FEV₁ and FVC changes.In the venous blood of patients with pulmonary fibrosis,the levels of TNF-α,TFG-β₁ and IL-6 promote the process of pulmonary fibrosis and are closely related to changes in respiratory function impairment.