Study On The Mechanism Of Zhiyan Xiaoji Prescription In The Treatment Of Liver Cancer Based On Network Pharmacology

Objective: To study the effect of Zhiyan Xiaoji Prescription on the biological behaviour of Hep G2 cells in liver cancer and to predict its main components,potential targets and mechanism of action using a network pharmacology approach.Methods: After different concentrations of Zhiyan xiaoji recipe aqueous extract were applied to human liver cancer Hep G2 cells,the proliferation ability of the cells was detected using CCK8 assay;the migration ability of the cells was detected by scratch and Transwell migration assay;Transwell invasion assay was used to detect the invasion ability of the cells;and the effect on cell cycle was detected by flow cytometry.TCMSP database and BATMAN-TCM database were used to collect the components and targets of Zhiyan Xiaoji Prescription.Genecards database collected the targets of liver cancer.The common targets of Zhiyan Xiaoji Prescription in the treatment of liver cancer were obtained by crossing the targets of Zhiyan Xiaoji Prescription and liver cancer.Import common targets into String database for PPI network analysis;TCM,compounds and common targets were imported into Cytoscape to make "TCM-compound-target" network diagram;The common targets were imported into David database for GO and KEGG enrichment analysis,and the signal pathways obtained from KEGG enrichment analysis were verified.Results: CCK8 showed that Zhiyan xiaoji recipe aqueous extract inhibited the proliferation of human hepatocellular carcinoma Hep G2 cells in a concentration-and time-dependent manner;the results of scratch and Transwell migration assays showed that Zhiyan xiaoji recipe aqueous extract inhibited the migration of human hepatocellular carcinoma Hep G2 cells;the results of Transwell invasion assay showed that Zhiyan xiaoji recipe aqueous extract inhibited the invasion of human hepatocellular carcinoma Hep G2 cells.The results of the Transwell invasion assay showed that Zhiyan xiaoji recipe aqueous extract inhibited the invasion of human hepatocellular carcinoma Hep G2 cells.TCMSP database and BATMAN-TCM database collected 154 compounds,1210 targets,Genecards database collected 139 targets for liver cancer,combined with intersection,61 common targets were obtained.It showed that the key targets were TP53,MYC,AKT1,CASP3,etc.The enrichment analysis showed that the main pathways involved were cancer pathway,apoptosis,p53 signalling pathway,PI3K-Akt signalling pathway and MAPK signalling pathway.The results of Western blot assay showed that Zhiyan xiaoji recipe aqueous extract could significantly down-regulate the expression of PI3 K,AKT and m TOR protein amounts.Conclusions: Zhiyan xiaoji recipe aqueous extract can inhibit the proliferation,migration,invasion and apoptosis of human hepatocellular carcinoma Hep G2 cells.Zhiyan Xiaoji Prescription on hepatocellular carcinoma involved multiple signaling pathways,and the aqueous extract of Zhiyan xiaoji recipe was able to down-regulate the expression of PI3 K,AKT and m TOR protein amounts.