Ultrastructural Observation And Clinical Effect Analysis Of MEBT/MEBO In Treating Chronic Refractory Wounds

Objective: To evaluate the clinical efficacy of skin regenerative medical technology(MEBT/MEBO)in treating chronic refractory wounds by comparing and observing the wound exudation,granulation growth,pain score,wound healing rate and scanning electron microscope results of wound tissue.Methods: Using random number method,100 patients with chronic refractory wounds who met the inclusion criteria were divided into treatment group and control group,with 50 patients in each group.The name,sex,age,wound site,wound area and depth,and basic disease of the patients in the group were recorded.The treatment group treated the wound with MEBT/MEBO for dressing change,and the control group treated the wound with recombinant bovine basic fibroblast growth factor gel(Bifuxin)for clinical experimental observation.The wound conditions of patients in the two groups were observed before treatment and on the 7th,14 th,21st and 28 th days of treatment.The wound tissues of 1 unhealed patient in the MEBT/MEBO group and 1 unhealed patient in the Bifuxin group were taken28 days after dressing change for scanning electron microscope observation,Observe the growth of granulation tissue,tissue arrangement,skin structure recovery,and evaluate the clinical efficacy of MEBT/MEBO by combining the wound exudation,granulation growth,pain score,wound healing rate and the results of scanning electron microscopy of wound tissue.Results: 1.For the wound tissue not healed after 28 days of MEBT/MEBO treatment,under the scanning electron microscope,a large number of fine and curly collagen fibers can be seen on the surface,with alternating light and dark periodic transverse lines,forming a relatively dense grid structure.The section can see thick and curly collagen fiber bundles of connective tissue,and the collagen fiber bundles form a relatively loose space structure,with new capillaries between the bundles,and mature red blood cells between the tissues,Scattered immature middle and late erythroblasts can be seen.The scanning electron microscope observation of the wound tissue that was not healed after 28 days of treatment with Befuxin showed that the fibers were arranged loosely and irregularly on the surface,scattered lymphocytes were seen between the fibers,and more scattered loose fibers were still seen between the collagen fiber bundles of the connective tissue on the cross section,and immature medium and late erythroblasts were mainly seen between the tissues.2.(1)After 7 days of treatment,there was no statistically significant difference in wound exudation score and wound area between the two groups(P>0.05).The comparison of granulation score and pain score showed statistical significance on the 7th day(P<0.05),indicating that on the 7th day of treatment,the two groups of drugs reduced wound exudation and wound healing speed,and the treatment group had better effects than the control group in promoting benign granulation growth and reducing wound pain.(2)After 14 days of treatment,the wound exudation score,granulation score,and pain score showed statistical significance(P<0.05),while the wound healing area was not statistically significant.This indicates that on the 14 th day of treatment,the treatment group was superior to the control group in reducing wound exudation,alleviating wound pain,and promoting benign granulation growth.The wound healing speed of the two groups was comparable.(3)After 21 days of treatment,the comparison of wound exudation score and wound healing area showed statistical significance(P<0.05),while the wound pain score and granulation score showed no statistical significance(P>0.05),indicating that after 21 days of treatment,the treatment group had a better effect in reducing wound exudation and promoting wound healing speed than the control group,and had similar effects in reducing wound pain and promoting granulation tissue growth.(4)After 28 days of treatment,the wound scores of the two groups of patients were compared,and the exudation score and wound healing area were statistically significant(P<0.05).The wound pain score and granulation score were not statistically significant(P>0.05),indicating that the treatment group was superior to the control group in promoting wound healing speed and reducing wound exudation after 28 days of treatment.The relief of wound pain and promoting granulation tissue growth were comparable.(5)Overall efficacy evaluation,the cure rate of the treatment group was 27.7%,and the total effective rate was 91.5%;The cure rate of the control group was 18.8%,and the total effective rate was 81.3%.The overall efficacy evaluation of the two groups was better in the treatment group than in the control group,with a statistically significant difference(P<0.05).Conclusion(s):(1)MEBT/MEBO has a significant clinical effect in treating chronic refractory wounds,which can effectively reduce wound exudation,relieve wound pain,improve wound healing rate and accelerate the process of wound repair.(2)In the early stage of medication,MEBT/MEBO can better relieve the pain symptoms of patients’ wounds and relieve the pain of patients than Befosin.(3)MEBT/MEBO can better improve the spatial arrangement of connective tissue fibers,restore the cell and tissue structure in the wound,and promote the benign growth of granulation tissue.