| Objective: To observe the effect of moxibustion Zusanli on intestinal flora of aging rats,and to explore its possible mechanism of delaying aging.Methods: Forty SPF healthy male SD rats were randomly divided into blank group,model group,prevention group and treatment group,with 10 rats in each group.Subacute aging rat models were prepared by intraperitoneal injection of D-galactose in model group,treatment group and prevention group.In the blank group,rats were placed in supine position and fixed on the experimental table every morning for 5min/d for 42 d,without any other intervention.The model group was injected intraperitoneally with D-galactose solution every morning,then placed in supine position and fixed on the experimental table,5min/d,and continuous 42 d modeling was made.In the prevention group,moxibustion and moxibustion were carried out after modeling every morning,and Zusanli acupoint was taken,once a day,each time for 3 consecutive times,continuous modeling and treatment 42 d.The treatment group was treated the next day after the successful modeling,and the moxibustion method and strength were the same as the prevention group.The contents of superoxide dismutase(SOD)and malondialdehyde(MDA)in serum were determined by enzyme-linked immunosorbent assay(ELISA).16 Sr RNA sequencing technology was used to detect the intestinal flora of each group after intervention.Results:(1)Compared with blank group,the serum SOD content of rats in model group was significantly decreased(P<0.01);Compared with model group,the serum SOD content of rats in prevention group and treatment group was significantly increased(P<0.01).Compared with the treatment group,the serum SOD content of the prevention group was significantly higher than that of the treatment group(P<0.01).(2)Compared with blank group,the content of MDA in serum of model group was significantly increased(P<0.01);Compared with model group,MDA content in serum of prevention group and treatment group was significantly decreased(P<0.01).Compared with the treatment group,MDA content in the prevention group was lower than that in the treatment group(P<0.05).(3)Compared with blank group,Chao1 index and Shannon index of model group decreased significantly(P<0.01),while Simpson index of model group had no significant difference with blank group(P>0.05).Compared with model group,Chao1 and Shannon indexes of rats in treatment group were increased(P<0.01,P<0.05),and those in prevention group were increased(P<0.01).Simpson index of rats in treatment group and prevention group was not significantly different from that in model group(P>0.05).There was no significant difference in Chao1,Shanno and Simpson indexes between prevention group and treatment group(P>0.05).(4)At the level of intestinal flora: 1.Compared with blank group,the relative abundance of Firmicutes in model group was significantly increased(P<0.01),while the relative abundance of Bacteroidetes was significantly decreased(P<0.01);Compared with model group,the relative abundance of Firmicutes in treatment group and prevention group was significantly decreased(P<0.01),while the relative abundance of Bacteroidetes was significantly increased(P<0.01).There was no significant difference in the relative abundance of microflora level between the prevention group and the treatment group(P>0.05).2.Compared with blank group,B/F value in model group was significantly decreased(P<0.01);Compared with model group,B/F value in treatment group and prevention group was significantly increased(P<0.01).There was no significant difference in B/F value between prevention group and treatment group(P>0.05).(5)At the level of intestinal flora: compared with blank group,the relative abundance of Lactobacillus and Prevotella UCG-003 in model group was decreased(P<0.05,P<0.01),while the relative abundance of Treponemia＿2 and Spirillaceae NK4A136 was significantly increased(P<0.01).Compared with model group,the relative abundance of Lactobacillus and Prevotella UCG-003 in treatment group and prevention group was significantly increased(P<0.01),while the relative abundance of Treponemia＿2 was significantly decreased(P<0.01).There was no significant difference between the prevention group and the treatment group in the relative abundance of flora genus level(P>0.05).Conclusion(s):Moxibustion Zusanli can increase the serum SOD content and reduce the serum MDA content of aging rats,regulate the diversity of intestinal flora and improve the structure of intestinal flora of aging rats,indicating that moxibustion Zusanli can improve the level of oxidative stress in aging rats and play a role in delaying aging.The mechanism may be related to the regulation of the diversity and structure of intestinal flora. |
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