| Background: Gastric cancer is a significant disease that poses a threat to human health.It is the fifth most common cancer and the fourth leading cause of cancer-related deaths worldwide.Exploring the mechanisms underlying the development and progression of gastric cancer and identifying effective treatment strategies are of great clinical significance for controlling its progression and improving the prognosis of patients.GLIS3 is a member of the zinc finger protein family,and its abnormal expression has been linked to several congenital diseases,such as polycystic kidney disease and congenital hypothyroidism.GLIS3 also plays a role in the development of malignant tumors,such as gliomas and breast cancer.However,the study of GLIS3 in gastric cancer has been limited to bioinformatics analysis and lacks clinical experimental data to support its findings.Objective: This study aims to analyze the expression of GLIS3 in gastric cancer tissues and its clinical significance in the prognosis of gastric cancer patients.Methods: Transcriptional expression profiles of GLIS3 between gastric cancer tissues and normal tissues were downloaded from the Cancer Genome Atlas(TCGA).Clinical data was collected from 61 patients diagnosed with gastric adenocarcinoma who underwent surgical treatment at Taizhou People’s Hospital from January 2018 to December 2018.The corresponding cancer tissue and adjacent tissue samples were collected and subjected to immunohistochemistry staining.The SPSS 27.0 statistical software was used to analyze the expression of GLIS3 protein in gastric cancer tissue and adjacent tissues.The follow-up of 61 patients was conducted to determine whether GLIS3 protein expression was a prognostic factor for gastric cancer.Results: Bioinformatics analysis revealed that the expression level of GLIS3 m RNA was significantly higher in gastric cancer tissue than in normal tissue.Moreover,the expression level of GLIS3 m RNA was associated with lymph node metastasis in gastric cancer tissue.Kaplan-Meier survival curve analysis showed that the overall survival of patients with high expression of GLIS3 m RNA was significantly worse,indicating that it may serve as a biomarker for poor prognosis in gastric cancer.In the study of correlation between the prognosis of gastric cancer patients and the expression of GLIS3,it was found that patients with high expression of GLIS3 protein had a lower overall survival rate than those with low expression,and the difference was statistically significant(P<0.05).The results of the COX proportional hazards model univariate analysis showed that whether the tumor had lymph node metastasis,tumor differentiation degree,postoperative chemotherapy status,and the expression level of GLIS3 all had an impact on prognosis.Multivariate analysis suggested that lymph node metastasis,tumor differentiation degree,and the expression level of GLIS3 protein were all independent factors affecting the prognosis of gastric cancer patients.Conclusion: 1.Through bioinformatics analysis,it was found that the expression of GLIS3 gene was upregulated in gastric cancer tissues and correlated with lymph node metastasis and poor prognosis.2.Experimental research found that the expression of GLIS3 protein was higher in gastric cancer tissues compared to adjacent noncancerous tissues.3.High expression of GLIS3 protein was correlated with factors such as tumor differentiation degree,lymph node metastasis,and vascular invasion in 61 gastric cancer patients included in the study.4.The expression of GLIS3 protein in gastric cancer tissues may be a predictor of patient prognosis,with elevated expression levels being indicative of a poor prognosis. |
PDF Full Text Request