| ObjectiveTo investigate the changes in cholesterol efflux mediated by adenosine triphosphate binding cassette G1(ABCG1)in patients with Systemic lupus erythematosus(SLE)and its clinical significance,and to investigate the effect of resveratrol(RSV)on the rate of ABCG1-mediated cholesterol efflux in this group of patients.The effect of RSV on the rate of ABCG1-mediated cholesterol efflux in patients with SLE.Methods73 patients with clearly diagnosed SLE(SLE group,n=73)were selected,including40 patients with combined cardiovascular complications(SY group,n=40)and 33 patients without combined cardiovascular complications(SN group,n=33).52 gender and age matched healthy physical examiners were selected as healthy controls(N group,n=52),and 50 patients with coronary heart disease,hypertension and other cardiovascular system diseases without the combination of SLE and other autoimmune diseases were selected as the disease control group(Group P,n=50).The lipid indexes of each group were measured and the changes in lipids were analyzed.ABCG1 plasmid vector was constructed and transfected with Chinese hamster ovary cells(CHO-K1/ABCG1),coincubated with serum from treated SLE patients,intervened with RSV at different concentrations(0μM,10μM,25μM,50μM,100μM),detected cholesterol efflux in each group by fluorescent labelling method,calculated cholesterol efflux rate,and statistically analysed the change in ABCG1.The cholesterol efflux rate was calculated,and the changes of cholesterol efflux mediated by ABCG1 and the intervention effect of RSV in each group were statistically analyzed.Results1.ABCG1 plasmid vector was constructed and transfected with CHO-K1 cells.2.Lipid test results: compared with the N group,TG and LDL were significantly higher(p<0.05)and HDL was significantly lower(p<0.05)in the SY group,N-HDL was significantly higher(p<0.05)in the SLE and SY groups;compared with the SN group,TG was significantly higher(p<0.05)and N-HDL was significantly lower(p<0.05)in the SY group,TC,HDL,and LDL were not significantly different(p>0.05).3.Cholesterol efflux assay results: In CHO-K1/ ABCG1 cells,the cholesterol efflux rate was significantly increased in the N,P,SLE,SY and SN groups compared to CHOK1 cells(p<0.05).In CHO-K1 cells,the cholesterol efflux rate was significantly higher in the SLE,SY and SN groups compared to the N and P groups(p<0.05);In CHOK1/ABCG1 cells,the cholesterol efflux rate was significantly higher in the SLE,SY and SN groups compared to the N group(p<0.05);Compared to the P group,the cholesterol efflux rate was significantly higher in the SLE and SY groups(p<0.05);Compared to the P group,the cholesterol efflux rate was significantly higher in the SLE and SY groups(p<0.05).The rate of cholesterol efflux was significantly higher in the SLE and SY groups compared to the P group(p<0.05).4.Effect of resveratrol on cholesterol efflux:(1)In CHO-K1 cells,the cholesterol efflux rate was significantly higher in the SLE,SY and SN groups compared to the N group(RSV: 10 μM,25 μM,50 μM,100 μM,p<0.05);Compared to the P group,the cholesterol efflux rate was significantly higher in the SLE,SY and SN groups(RSV: 25 μM,100 μM,p<0.05).The cholesterol efflux rate was significantly higher in both the SLE and SY groups(RSV: 10 μM,p<0.05)and in the SY group(RSV: 50 μM,p<0.05).(2)In CHO-K1/ABCG1 cells,the cholesterol efflux rates were significantly higher in the SLE,SY and SN groups compared to the N group(RSV: 10 μM,25 μM,50 μM,p<0.05),and in the SLE and SN groups(RSV: 100 μM,p<0.05);Compared to the P group,the cholesterol efflux rates in the SY group(RSV: 10μM,25μM,50μM,p<0.05)and the cholesterol efflux rate was significantly higher in both the SLE and SN groups(RSV:100μM,p<0.05)compared to the P group.(3)In CHO-K1 cells,compared with 0 μM RSV,10 μM RSV had no significant effect on cholesterol efflux in all groups,and as RSV concentration increased,the cholesterol efflux rate in all groups was negatively correlated with RSV concentration(p<0.05);In CHO-K1/ABCG1 cells,the cholesterol efflux rate in the N,P,SLE and SY groups was negatively correlated with RSV concentration(p<0.05),and no correlation between the SN group and RSV concentration(p>0.05).Conclusion1.Significant changes in TG,LDL,HDL and N-HDL occur in SLE patients,especially in patients with combined cardiovascular complications.2.ABCG1-mediated cholesterol efflux is significantly altered in SLE patients and may have a relationship with the high prevalence of cardiovascular disease in SLE patients.3.In the concentration range used in this experiment,low concentrations of RSV had no significant effect on cholesterol efflux in all groups,and as the concentration of RSV increased,the rate of cholesterol efflux was negatively correlated with its concentration. |
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