Studies On The Chemical Constituents Of Tripterygium Regelii And Anti-Inflammatory Activity

Objective:The study investigates and identifies structures about the chemical constituents of the roots of Tripterygium regelii,making the anti-inflammatory activity evaluation of the isolated compounds.Methods:The roots of Tripterygium regelii are extracted with 70%ethanol..The chemical constituents of Tripterygium regelii ethyl acetate extract were isolated by a variety of chromatographic techniques such as silica gel column,ODS,HPLC,and pre-HPLC.And their structures are elucidated based on a variety of spectral analysis techniques such as ¹H NMR,¹³CNMR,2D NMR,X-ray crystallography and MS,etc.The Griess method is used to observe the effect of No formed by mouse monocyte-macrophage RAW264.7,for assessing the anti-inflammatory activity of the chemical constituents.Results:Sixty nine compounds（1-69）are isolated from the roots of Tripterygium regelii by adopting spectral analysis techniques such as 1D NMR,2D NMR,HRESIMS combined with methods such as X-ray crystallography,which clarifies their structure,including 18 new compounds（1*-18*）.,The serial numbers and the corresponding names about isolation and identification of compounds are shown in Table 1.The activity of all isolated compounds is made on LPS-induced NO production in mouse monocyte-macrophage RAW 264.7.As a result,based on compounds 16,17,18,33,39,43,67 and 69（100μM）,their inhibiting rates on the NO production of macrophage is over 80%.And,compound 16 has a 96%inhibiting rate on the NO production.Conclusion:The investigation is mainly concerning about the isolation,structural identification and anti-inflammatory activity evaluation of chemical constituents of the roots of Tripterygium regelii.The isolated and identified compounds include 10sesquiterpenoids（14-15、19-26）,19 diterpenoids（16-17,27-43）,3 triterpenoids（18、44-45）,25 alkaloids（1-13、46-57）,1 lignin（59）,2 coumarins（60-61）and other 9compounds（58、62-69）.Diterpenoids and macrolide alkaloids of Tripterygium regelii show a significant anti-inflammatory activity,which provides a reference for modern pharmacological application of Tripterygium regelii.