| ObjectiveTo study the protective effect and mechanism of Astragaloside IV(AS-IV)on rats with cerebral ischemia reperfusion injury(CIRI).MethodsNinety healthy male SD(Sprague-Dawley,SD)rats weighing 250-280 g were randomly divided into 3 groups: Sham group,MCAO group,and(AS-IV 30mg/kg)group.The MCAO group and AS-IV group underwent middle cerebral artery cerebral ischemia-reperfusion model(2 hours of middle cerebral artery occlusion and 24 hours reperfusion),and the Sham group was given the same dose of normal saline.1.Zea-Longa neurobehavioral score method was used to detect neurological deficits in rats.2.The change of cerebral infarction volume in rats was detected by 2,3,5-triphenyltetrazolium chloride(TTC)staining.3.SOD and MDA kits were used to determine the level of oxidative stress in brain tissue.4.HE staining was used to observe the histopathological morphological changes of rat brain.5.Immunohistochemical methods were used to determine the expression of Nrf2,HO-1,Bcl-2 and Bax proteins in brain tissues.6.Western blotting was used to detect the expression of Nrf2,HO-1,Bcl-2 and Bax proteins in brain tissues.Results1.The results of neurobehavioral score showed that: rats in the Sham group did not show symptoms of neurological deficits;Rats in the MCAO group had obvious symptoms of neurological deficit and significantly increased scores(P<0.05),while compared with the MCAO group,the neurobehavioral scores of the AS-IV group decreased significantly(P<0.05).2.The TTC staining results showed that: the volume of cerebral infarction in rats in the Sham group was 0;The volume of infarct in rats in the MCAO group increased significantly(P<0.05).The volume of cerebral infarction in the AS-IV group was reduced compared with that in the MCAO group(P<0.05).3.The results of SOD activity and MDA content determination showed that: compared with the Sham group,the SOD activity of rats in the MCAO group decreased(P<0.05)and the MDA content increased significantly(P<0.05).Compared with the MCAO group,the AS-IV group showed increased SOD activity(P<0.05)and decreased MDA content(P<0.05).4.The results of HE staining showed that: the cell morphology of the cerebral cortex area of the rats in the Sham group was regular,the nucleoli outline was clear,and the cells were closely arranged;Compared with the Sham group,the rats in the MCAO group had obvious pathological damage to the ischemic cortical area,deep nuclear staining and solidification,increased intercellular space,obvious cavities,and shallow cytoplasmic staining.Compared with the MCAO group,the injury caused by ischemia-reperfusion in the AS-IV group was significantly improved.5.The Immunohistochemical results showed that: compared with the Sham group,the positive expression of Nrf2,HO-1 and Bax in the MCAO group increased(P<0.05)and the positive expression of Bcl-2 decreased(P<0.05).Compared with the MCAO group,the positive expression levels of Nrf2,HO-1 and Bcl-2 were increased in the AS-IV group(P<0.05)and the positive expression level of Bax(P<0.05)decreased.6.The results of Western blotting showed that: compared with the Sham group,the levels of Nrf2,HO-1 and Bax proteins in the MCAO group increased(P<0.05)and the levels of Bcl-2 proteins decreased(P<0.05).Compared with the MCAO group,the expression levels of Nrf2,HO-1 and Bcl-2 proteins in the ASIV group increased(P<0.05)and the levels of Bax proteins decreased(P<0.05).ConclusionsAstragaloside IV has a protective effect on cerebral ischemia-reperfusioninjured rats,and its mechanism of action may be related to the upregulation of Nrf2,HO-1,Bcl-2 protein expression and down-regulation of Bax protein expression. |
PDF Full Text Request