| ObjectiveInvestigating the protective effects of taurine on rat brain ischemiareperfusion injury(IR)through anti-oxidative stress and anti-apoptosis.MethodsSelected to construct a rat brain ischemia-reperfusion model were healthy SPF-grade male SD rats with body masses ranging from 250g-280 g,randomly divided into three groups: Sham-operated,IR,and TAU.Following two hours of cerebral ischemia and 24 hours of reperfusion,the rats in each group were evaluated for neurological deficits,with those scoring 3-6 being deemed successful in modelling.Those that failed were excluded,while those that passed were chosen as study subjects.Afterward,TTC staining was done to gauge the magnitude of cerebral infarction;SOD and MDA kits were utilized to detect oxidative stress in brain tissue;HE and Nissl staining was employed to detect pathological alterations in cerebral cortical areas;TUNEL kits were utilized to detect apoptosis in ischemic regions;and Immunofluorescence and immunoblotting revealed the expression of PI3 K,AKT,Cleaved Caspase-3 and Bcl-2 proteins.Finally,correlation analysis was performed.ResultsCompared with the Sham group:(1)The neurological deficit score was significantly higher and the infarct volume of brain tissue was significantly larger in the IR group;after TAU treatment,the neurological deficit score was lower and the infarct volume of brain tissue was smaller.(2)Superoxide dismutase(SOD)level was significantly decreased and malondialdehyde(MDA)content was significantly increased in the IR group;after TAU treatment,superoxide dismutase(SOD)level was increased and malondialdehyde(MDA)content was decreased.(3)HE staining showed that the brain tissue structure of the IR group was obviously loose and damaged,the nerve cells were irregularly arranged,the nuclei were pyknotic,and even some nuclei were lysed.In Tau-treated rats,the cortical damage was alleviated,the nerve cells were arranged in an orderly manner,and the nuclear pyknosis was also significantly improved.(4)Nissl staining showed that the nucleus membrane and nucleolus of neurons in the IR group were blurred,the protrusions were discontinuous,and the cytoplasmic nucleolus was lighter in color and fewer;however,TAU-treated rats’ neurons’ nucleolus and membrane in brain tissue were distinctly distinguishable,with the cytoplasmic nucleolus partially lost,though it was significantly less than the IR group’s.(5)TUNEL staining observed a significant increase in the number of apoptotic cells in the cortical area of the rats in the IR group;after TAU treatment,the positive apoptosis was significantly reduced.(6)Immunofluorescence staining showed that the fluorescence expression of PI3 K and AKT in the brain tissue of the rats in the IR group was significantly reduced;the fluorescence expression of PI3 K and AKT in the brain tissue of the rats after TAU treatment was increased.(7)Western-blot showed that the expression of PI3 K and AKT in the brain tissues of the rats in the IR group was significantly reduced,while the expression of PI3 K and AKT in the rats after TAU treatment was increased.(8)Western-blot detection of apoptosis-related proteins showed that the expression of Cleaved Caspase-3 was significantly increased and that of Bcl-2 was significantly decreased in the brain tissues of rats in the IR group;the expression of Cleaved Caspase-3 protein was decreased in the brain tissues of rats after TAU treatment;the expression of protein in Bcl-2 was increased The expression of Cleaved Caspase-3 protein in rat brain tissues was reduced after TAU treatment.ConclusionsTaurine plays an anti-oxidative stress role by increasing the activity of SOD and reducing the content of MDA in the brain tissue of rats.Taurine plays an antimitochondrial apoptosis signal mediated apoptosis effect by promoting the expression of Bcl-2,PI3 K and AKT,and inhibiting the expression of CleavedCaspase3,and may activate the PI3K/AKT signaling pathway.It plays a protective role in cerebral ischemia-reperfusion injury. |
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