Diagnostic Efficacy Of Stool Genes SDC2 And SFRP2 Methylation In Colorectal Cancer Screening

ObjectiveTo investigate the screening value and diagnostic efficacy of fecal gene heparin sulfate proteoglycan 2(SDC2)combined with secreted frizzled-related protein 2(SFRP2)methylation detection in patients with colorectal cancer,and to provide reference for clinical diagnosis and treatment.MethodsFifty-one patients with colorectal cancer admitted to the Department of General Surgery or gastroenterology of the First Affiliated Hospital of Qiqihar Medical College from January 2021 to September 2021 were selected as the observation group.Sixty-one patients with advanced adenoma treated at the same time were selected as control group 1.Fifty healthy subjects in the same period were selected as control group 2.Fecal samples were collected from each group,and the methylation of SDC2 and SFRP2 in feces was determined by methylation-specific PCR,and the methylation of SDC2 and BMP3 in feces in each group was compared.Pathological data of colorectal cancer patients were reviewed,gender,age,lesion site,tumor size,degree of differentiation and TNM stage of patients were analyzed,and the positive rates of SDC2 and SFRP2 methylation in feces under different pathological conditions were analyzed,and multivariate Logistic regression analysis was performed.ROC curves were drawn to analyze the efficacy(sensitivity and specificity)of fecal SDC2 and SFRP2 methylation in colorectal cancer screening.All data were processed by SPSS26.0 software.Fifty healthy subjects in the same period were selected as control group 2.Fecal samples were collected from each group,and the methylation of SDC2 and SFRP2 in feces was determined by methylation-specific PCR,and the methylation of SDC2 and SFRP2 in feces in each group was compared.Pathological data of colorectal cancer patients were reviewed,gender,age,lesion site,tumor size,degree of differentiation and TNM stage of patients were analyzed,and the positive rates of SDC2 and SFRP2 methylation in feces under different pathological conditions were analyzed,and multivariate Logistic regression analysis was performed.ROC curves were drawn to analyze the efficacy(sensitivity and specificity)of fecal SDC2 and SFRP2 methylation in colorectal cancer screening.All data were processed by SPSS26.0 software.Fifty healthy subjects in the same period were selected as control group 2.Fecal samples were collected from each group,and the methylation of SDC2 and SFRP2 in feces was determined by methylation-specific PCR,and the methylation of SDC2 and SFRP2 in feces in each group was compared.Pathological data of colorectal cancer patients were reviewed,gender,age,lesion site,tumor size,degree of differentiation and TNM stage of patients were analyzed,and the positive rates of SDC2 and SFRP2 methylation in feces under different pathological conditions were analyzed,and multivariate Logistic regression analysis was performed.ROC curves were drawn to analyze the efficacy(sensitivity and specificity)of fecal SDC2 and SFRP2 methylation in colorectal cancer screening.All data were processed by SPSS26.0 software.Results(1)The methylation rate of SDC2 and SFRP2 in feces in observation group was higher than that in control group 1 and control group 2(P<0.02).The methylation rate of SDC2 and SFRP2 in feces in control group 1 was higher than that in control group 2(P<0.02).Excrement gene SDC2 and SFRP2 joint detection sensitivity is higher than single gene detection,the difference was statistically significant(P < 0.01).(2)There were no statistical differences between SDC2 and SFRP2 methylation in feces of colorectal cancer patients and gender,age,tumor size and lesion site(P>0.05).There were statistical differences with differentiation type and TNM stage(P<0.05).TNM stage(β=1.213,OR=3.582,95%CI =2.482-6.313)and differentiation degree(β=1.563,OR=6.413,95%CI =5.682-8.452)were factors influencing the positive rate of SDC2 and SFRP2 methylation in feces of patients with colorectal cancer.(3)ROC curve results showed that the diagnostic sensitivity and specificity of fecal SDC2 and SFRP2 methylation in colorectal cancer patients were higher than that of single fecal SDC2 and SFRP2 methylation determination(P<0.05),and fecal SDC2 and SFRP2 methylation also had higher diagnostic efficacy in colorectal cancer patients.TNM stage(β=1.213,OR=3.582,95%CI=2.482-6.313)and differentiation degree(β=1.563,OR=6.413,95%CI=5.682-8.452)were factors influencing the positive rate of SDC2 and SFRP2 methylation in feces of patients with colorectal cancer.(3)ROC curve results showed that the diagnostic sensitivity and specificity of fecal SDC2 and SFRP2 methylation in colorectal cancer patients were higher than that of single fecal SDC2 and SFRP2 methylation determination(P<0.05),and fecal SDC2 and SFRP2 methylation also had higher diagnostic efficacy in colorectal cancer patients.TNM stage(β=1.213,OR=3.582,95%CI =2.482-6.313)and differentiation degree(β=1.563,OR=6.413,95%CI =5.682-8.452)were factors influencing the positive rate of SDC2 and SFRP2 methylation in feces of patients with colorectal cancer.(3)ROC curve results showed that the diagnostic sensitivity and specificity of fecal SDC2 and SFRP2 methylation in colorectal cancer patients were higher than that of single fecal SDC2 and SFRP2 methylation determination(P<0.05),and fecal SDC2 and SFRP2 methylation also had higher diagnostic efficacy in colorectal cancer patients.Conclusions(1)Fecal SDC2 and SFRP2 methylation were highly expressed in both colorectal cancer and advanced adenoma patients,and the positive rate of colorectal cancer was higher than that of advanced adenoma patients.(2)The expression levels of SDC2 and SFRP2 methylation in feces in colorectal cancer patients were correlated with the stage and differentiation of TNM tumors;(3)Fecal SDC2 combined with SFRP2 methylation can obtain high diagnostic sensitivity and specificity in colorectal cancer,can guide clinical diagnosis and treatment,and is worthy of clinical application.The expression levels of SDC2 and SFRP2methylation in feces in colorectal cancer patients were correlated with the stage and differentiation of TNM tumors;(3)Fecal SDC2 combined with SFRP2 methylation can obtain high diagnostic sensitivity and specificity in colorectal cancer,can guide clinical diagnosis and treatment,and is worthy of clinical application.