Zinc Ions Regulate Neutrophils And Inflammatory Response By Activating The IL-17 Signaling Pathway To Promote Functional Recovery In Spinal Cord Injury

Objective The objective of this study was to investigate the molecular mechanism of zinc ions in treating spinal cord injury and to provide a research basis for the clinical use of zinc ions in this regard.Methods To investigate the molecular mechanism of zinc ions in treating spinal cord injury,we divided mice into two groups: spinal cord injury group and post-spinal cord injury zinc ion treatment group.We used single-cell sequencing technology to sequence the transcriptome.We further divided the mice into five groups:control group,sham-operated group,spinal cord injury group,spinal cord injury zinc ion treatment group,and IL-17 pathway inhibition group.We employed PCR-Array,tissue immunofluorescence,western blot,and q RT-PCR to validate the experiments.Results1、Zinc ion therapy for spinal cord injury in mice showed better functional recovery,and sequencing results showed the activation of the IL-17 signaling pathway.2、The use of gene chip technology successfully validated the activation of the IL-17 signaling pathway by zinc ion therapy in animals.The effect of the activated IL-17 signaling pathway by zinc ion therapy was an increase in neutrophil aggregation and a decrease in the expression of inflammatory factors at the site of spinal cord injury.3、Inhibition of the IL-17 signaling pathway in the zinc ion therapy group weakened the phenomenon of functional recovery,increased neutrophil aggregation,and reduced expression of inflammatory factors caused by zinc ion therapy.4 、 Single-cell sequencing of neutrophils in the site of spinal cord injury suggested immunoheterogeneity of neutrophils in the zinc ion therapy groupConclusions1、Zinc ion therapy promotes non-classical activation of the IL-17 pathway after spinal cord injury.2 、 Zinc ion therapy promotes immunoheterogeneity of neutrophils after spinal cord injury.3、Zinc ion therapy can promote spinal cord injury recovery through the above-mentioned ways.