Expression Of IL-33 And Its Receptor ST2L In Peripheral Blood T Cells And Skin Lesions In Patients With Psoriasis

ObjectiveTo observe the expression of Interleukin 33(IL-33)in peripheral blood T cells and skin lesions of patients with vulgaris and pustule psoriasis.Meanwhile,the correlation between Interleukin 17A(IL-17A)and IL-33 expression level in peripheral blood T cells of patients with vulgaris and pustule psoriasis was observed,and the effects of IL-33 and its receptor ST2 L on the occurrence,development and severity of psoriasis vulgaris and pustule psoriasis were explored.MethodsA total of 40 patients with psoriasis were collected in this study,including 20 patients with psoriasis vulgaris(plaque type)(PV group)and 20 patients with psoriasis pustulosa(PP group).In addition,20 patients who underwent nevus resection were selected as control group(CON group).Gender,age,time of onset,family history of Psoriasis,history of upper respiratory tract infection before onset of psoriasis,Psoriasis Area and Severity of psoriasis were recorded in PV group and PP group Index(PASI score),Dermatology Life Quality Index(DLQI score);Gender and age of CON group were also recorded.Enzyme linked immunosorbent assay(ELISA)was used to detect the expression levels of IL-17 A and IL-33 in peripheral blood serum of the PV,PP and CON groups,and to detect the extracellular secretion levels of IL-33 after IL-17 A induced Ha Cat keratinocytes.Immunohistochemical staining was used to detect the expression of IL-33 and its receptor ST2 L in the lesions of the PV group,the lesions of the PP group and the non-lesions of the CON group.Western Blot(WB)was used to determine the expression level of IL-33 protein in Jurkat T cells and Ha Cat keratinocytes induced by IL-17 A,and the expression level of IL-33 protein in peripheral blood T cells of patients in PV group,PP group and CON group.IL-33 induced the expression of ST2 L protein in peripheral blood T cells of Jurkat T cells,Ha Cat keratinocytes,PV group,PP group and CON group.The expression levels of IL-33 protein and STAT3/p-STAT3 protein in peripheral blood T cells of patients in PV group,PP group and CON group were detected after induction by IL-17A(50ng/m L).Realtime-PCR was used to detect the IL-33 m RNA expression in Jurkat T cells and Ha Cat keratinocytes induced by IL-17 A,and the expression of IL-33 m RNA in peripheral blood T cells of patients in PV,PP and CON groups.The expression of ST2 Lm RNA in peripheral blood T cells of Jurkat T cells,PV group,PP group and CON group was detected by IL-33.Results1.IL-33 expression was found in peripheral blood serum and skin lesions of PV group,PP group and CON group,PP group > PV group > CON group,and the difference between the two groups was statistically significant(P < 0.05).IL-33 and its receptor ST2 L were expressed in skin lesions of PV group,PP group and CON group,PP group > PV group > CON group,and the difference between the two groups was statistically significant(P < 0.05).The expression level of IL-33 in peripheral blood serum of PV group and PP group was positively correlated with PASI score and DLQI score.The expression level of IL-17 A in peripheral blood serum of PV group and PP group was positively correlated with the expression level of IL-33.2.IL-17 A induced Jurkat T cells and Ha Cat keratinocytes to produce IL-33 protein and IL-33 m RNA,showing a certain amount of effect and aging relationship;IL-17 A induced the production of IL-33 protein and IL-33 m RNA by T cells in PV group,PP group and CON group,and showed a certain effect and aging relationship.IL-17A(50ng/m L)induced the production of IL-33 protein,STAT3 protein and p-STAT3 protein in peripheral blood T cells of patients in PV,PP and CON groups,PP group > PV group > CON group,and the difference was statistically significant(P < 0.05).IL-33 induced the production of ST2 L protein and ST2 Lm RNA in Jurkat T cells,Ha Cat keratinocytes,PV group,PP group and CON group,and showed a certain effect relationship.ConclusionIt was found that the expression level of IL-33 and its receptor ST2 L in peripheral blood T cells and skin lesions of patients with psoriasis was significantly increased compared with that of the control group,and it was related to the severity of psoriasis and induced activation of STAT3/p-STAT3 signaling pathway,which may have an important impact on the occurrence and development of psoriasis.